Free fatty acid uptake in humans with CD36 deficiency

Kazanna C. Hames, Adrian Vella, Bradley J. Kemp, Michael Dennis Jensen

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Animal models have demonstrated that CD36 facilitates cell membrane free fatty acid (FFA) transport, but its role in human metabolism is not well understood. We measured heart, liver, adipose (three depots), and muscle (truncal postural and thigh locomotive) FFA uptake using [11C]palmitate positron emission tomography (PET) scans in a family of five carrying the Pro90Ser CD36 mutation (2 homozygotes had no CD36) and matched control volunteers. PET scans were done under conditions of suppressed and slightly increased palmitate concentrations. During suppressed palmitate conditions, muscle and adipose palmitate uptake were markedly reduced in homozygotes but not heterozygotes for the Pro90Ser CD36 mutation, whereas when palmitate concentration was slightly increased, uptake in muscle and adipose did not differ between control subjects and homozygous family members. Hepatic FFA uptake was similar in all participants regardless of palmitate concentrations, whereas myocardial FFA uptake was diminished in the Pro90Ser homozygotes during both suppressed and increased palmitate conditions. We conclude that CD36 1) facilitates FFA transport into muscle and adipose tissue in humans when extracellular concentrations are reduced but not when they are modestly elevated, 2) is not rate limiting for hepatic FFA uptake, and 3) is needed for normal cardiac FFA uptake over a range of FFA concentrations from low to slightly elevated.

Original languageEnglish (US)
Pages (from-to)3606-3614
Number of pages9
JournalDiabetes
Volume63
Issue number11
DOIs
StatePublished - Nov 1 2014

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Palmitates
Nonesterified Fatty Acids
Homozygote
Muscles
Positron-Emission Tomography
Liver
Mutation
Platelet Glycoprotein IV Deficiency
Heterozygote
Thigh
Adipose Tissue
Volunteers
Animal Models
Cell Membrane

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Free fatty acid uptake in humans with CD36 deficiency. / Hames, Kazanna C.; Vella, Adrian; Kemp, Bradley J.; Jensen, Michael Dennis.

In: Diabetes, Vol. 63, No. 11, 01.11.2014, p. 3606-3614.

Research output: Contribution to journalArticle

Hames, Kazanna C. ; Vella, Adrian ; Kemp, Bradley J. ; Jensen, Michael Dennis. / Free fatty acid uptake in humans with CD36 deficiency. In: Diabetes. 2014 ; Vol. 63, No. 11. pp. 3606-3614.
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