Abstract
Low intravenous infusion rates of [U-13C]palmitate were used to measure systemic palmitate flux at rest (0.5 nmol · kg-1 · min-1) and during exercise (2 nmol · kg-1 · min-) in healthy adults. The 13C enrichment of plasma palmitate was determined by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRM8). Palmitate flux was also measured using palmitate radiotracers, which were infused simultaneously to serve as a reference method. Flux values obtained using the two different methods were virtually identical. Overnight postabsorptive palmitate flux was measured on four occasions in three individuals; the flux values were highly reproducible with a coefficient of variation of 11% and 12% for intra-day measurement, and 13% and 8% for inter- day measurement, for [1-14C]palmitate and [U-13C~palmitate, respectively. During exercise, palmitate turnover measured with [U- 13C]palmitate and [3H] palmitate were well correlated (τ = 0.91). Compared to conventional stable isotope approaches, (e.g., [1-13C]palmitate analyzed by GC/MS), [U-13C]palmitate reduces the tracer dose by two orders of magnitude when the samples are analyzed with GC/G/IRMS. Uniformly labeled [13C]palmitate is suitable for the measurement of free fatty acid flux in humans.
Original language | English (US) |
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Pages (from-to) | 1888-1895 |
Number of pages | 8 |
Journal | Journal of Lipid Research |
Volume | 38 |
Issue number | 9 |
State | Published - Sep 1997 |
Keywords
- Exer-cise
- Gas chromatography/combustion/iso-tope ratio mass spectrometry
- [H]palmitate
- []palmitate
ASJC Scopus subject areas
- Biochemistry
- Endocrinology
- Cell Biology