Free d-amino acids in human cerebrospinal fluid of alzheimer disease, multiple sclerosis, and healthy control subjects

George H. Fisher, Leonard Petrucelli, Christina Gardner, Carolyn Emory, William H. Frey, Luigi Amaducci, Sandro Sorbi, Giovanna Sorrentino, Mauro Borghi, Antimo D'aniello

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

This is the first report of the presence of free D-amino acids in lumbar and ventricular human cerebrospinal fluid (CSF) of individuals with Alzheimer disease (AD) compared with CSF of normal control subjects and with individuals affected by multiple sclerosis, as an unrelated neurologic disorder. Free d-amino acids are present at significantly higher levels in AD CSF than normal CSF, whereas in the CSF of patients affected by multiple sclerosis, d-amino acids occurs at the same level as in the normal controls. The total d-amino acid content in ventricular CSF was 1.48 times higher in the AD than controls (26.4 vs 17.9 nmol/mL, p=0.025). The total d-amino acid content was 1.43 times higher in AD lumbar CSF than controls (1.89 vs. 1.32 nmol/mL, p=0.001). d-Aspartate in particular was 2.74 times higher in AD ventricular CSF compared to normal ventricular CSF (3.34 vs 1.22 nmol/mL, p=0.029). In lumbar CSF, d-aspartate was 1.5 times higher in AD than controls (0.054 vs 0.036 nmol/mL, p=0.041). Previously we reported that d-amino acids are elevated in AD brain proteins associated with neurofibrillary tangles compared to normal brain proteins (D'Aniello et al., 1992c; Fisher et al., 1992a,b). Thus, the d-amino acids present in CSF may originate from degradation of brain proteins.

Original languageEnglish (US)
Pages (from-to)115-124
Number of pages10
JournalMolecular and Chemical Neuropathology
Volume23
Issue number2-3
DOIs
StatePublished - Oct 1994

Keywords

  • Alzheimer disease
  • aspartic acid
  • cerebrospinal fluid
  • d-Amino acids
  • lumbar CSF
  • multiple sclerosis
  • ventricular CSF

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology

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    Fisher, G. H., Petrucelli, L., Gardner, C., Emory, C., Frey, W. H., Amaducci, L., Sorbi, S., Sorrentino, G., Borghi, M., & D'aniello, A. (1994). Free d-amino acids in human cerebrospinal fluid of alzheimer disease, multiple sclerosis, and healthy control subjects. Molecular and Chemical Neuropathology, 23(2-3), 115-124. https://doi.org/10.1007/BF02815405