Abstract
Human basal-like breast cancer (BLBC) is an enigmatic and aggressive malignancy with a poor prognosis. There is an urgent need to identify therapeutic targets for BLBC, because current treatment modalities are limited and not effective. The forkhead box transcription factor FOXC1 has recently been identified as a critical functional biomarker for BLBC. However, how it orchestrates BLBC cells was not clear. Here we show that FOXC1 activates the transcription factor nuclear factor-κB (NF-κB) in BLBC cells by increasing p65/RelA protein stability. High NF-κB activity has been associated with estrogen receptor-negative breast cancer, particularly BLBC. The effect of FOXC1 on p65/RelA protein stability is mediated by increased expression of Pin1, a peptidyl-prolyl isomerase. FOXC1 requires NF-κB for its regulation of cell proliferation, migration and invasion. Notably, FOXC1 overexpression renders breast cancer cells more susceptible to pharmacological inhibition of NF-κB. These results suggest that BLBC cells may rely on FOXC1-driven NF-κB signaling. Interventions of this pathway may provide modalities for the treatment of BLBC.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4798-4802 |
| Number of pages | 5 |
| Journal | Oncogene |
| Volume | 31 |
| Issue number | 45 |
| DOIs | |
| State | Published - Nov 8 2012 |
| Externally published | Yes |
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Keywords
- Basal-like breast cancer
- FOXC1
- NF-κB
- p65/RelA
- Pin1
- Protein stability
ASJC Scopus subject areas
- Molecular Biology
- Cancer Research
- Genetics
Cite this
FOXC1 regulates the functions of human basal-like breast cancer cells by activating NF-κB signaling. / Wang, J.; Ray, P. S.; Sim, M. S.; Zhou, X. Z.; Lu, K. P.; Lee, A. V.; Lin, X.; Bagaria, S. P.; Giuliano, A. E.; Cui, X.
In: Oncogene, Vol. 31, No. 45, 08.11.2012, p. 4798-4802.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - FOXC1 regulates the functions of human basal-like breast cancer cells by activating NF-κB signaling
AU - Wang, J.
AU - Ray, P. S.
AU - Sim, M. S.
AU - Zhou, X. Z.
AU - Lu, K. P.
AU - Lee, A. V.
AU - Lin, X.
AU - Bagaria, S. P.
AU - Giuliano, A. E.
AU - Cui, X.
PY - 2012/11/8
Y1 - 2012/11/8
N2 - Human basal-like breast cancer (BLBC) is an enigmatic and aggressive malignancy with a poor prognosis. There is an urgent need to identify therapeutic targets for BLBC, because current treatment modalities are limited and not effective. The forkhead box transcription factor FOXC1 has recently been identified as a critical functional biomarker for BLBC. However, how it orchestrates BLBC cells was not clear. Here we show that FOXC1 activates the transcription factor nuclear factor-κB (NF-κB) in BLBC cells by increasing p65/RelA protein stability. High NF-κB activity has been associated with estrogen receptor-negative breast cancer, particularly BLBC. The effect of FOXC1 on p65/RelA protein stability is mediated by increased expression of Pin1, a peptidyl-prolyl isomerase. FOXC1 requires NF-κB for its regulation of cell proliferation, migration and invasion. Notably, FOXC1 overexpression renders breast cancer cells more susceptible to pharmacological inhibition of NF-κB. These results suggest that BLBC cells may rely on FOXC1-driven NF-κB signaling. Interventions of this pathway may provide modalities for the treatment of BLBC.
AB - Human basal-like breast cancer (BLBC) is an enigmatic and aggressive malignancy with a poor prognosis. There is an urgent need to identify therapeutic targets for BLBC, because current treatment modalities are limited and not effective. The forkhead box transcription factor FOXC1 has recently been identified as a critical functional biomarker for BLBC. However, how it orchestrates BLBC cells was not clear. Here we show that FOXC1 activates the transcription factor nuclear factor-κB (NF-κB) in BLBC cells by increasing p65/RelA protein stability. High NF-κB activity has been associated with estrogen receptor-negative breast cancer, particularly BLBC. The effect of FOXC1 on p65/RelA protein stability is mediated by increased expression of Pin1, a peptidyl-prolyl isomerase. FOXC1 requires NF-κB for its regulation of cell proliferation, migration and invasion. Notably, FOXC1 overexpression renders breast cancer cells more susceptible to pharmacological inhibition of NF-κB. These results suggest that BLBC cells may rely on FOXC1-driven NF-κB signaling. Interventions of this pathway may provide modalities for the treatment of BLBC.
KW - Basal-like breast cancer
KW - FOXC1
KW - NF-κB
KW - p65/RelA
KW - Pin1
KW - Protein stability
UR - http://www.scopus.com/inward/record.url?scp=84869088684&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84869088684&partnerID=8YFLogxK
U2 - 10.1038/onc.2011.635
DO - 10.1038/onc.2011.635
M3 - Article
C2 - 22249250
AN - SCOPUS:84869088684
VL - 31
SP - 4798
EP - 4802
JO - Oncogene
JF - Oncogene
SN - 0950-9232
IS - 45
ER -