TY - JOUR
T1 - Forced expression of essential myosin light chain isoforms demonstrates their role in smooth muscle force production
AU - Huang, Qi Quan
AU - Fisher, Steven A.
AU - Brozovich, Frank V.
PY - 1999/12/3
Y1 - 1999/12/3
N2 - The molecular determinants of the contractile properties of smooth muscle are poorly understood, and have been suggested to be controlled by splice variant expression of the myosin heavy chain near the 25/50-kDa junction (Kelley, C. A., Takahashi, M., Yu, J. H., and Adelstein, R. S. (1993) J. Biol. Chem. 268, 12848-12854) as well as by differences in the expression of an acidic (MLC(17a)) and a basic (MLC(17b)) isoform of the 17- kDa essential myosin light chain (Nabeshima, Y., Nonomura, Y., and Fujii- Kuriyama, Y. (1987) J. Biol. Chem. 262, 10650810612). To investigate the molecular mechanism that regulates the mechanical properties of smooth muscle, we determined the effect of forced expression of MLC(17a) and MLC(17b) on the rate of force activation during agonist-stimulated contractions of single cultured chicken embryonic aortic and gizzard smooth muscle cells. Forced expression of MLC(17a) in aortic smooth muscle cells increased (p < 0.05) the rate of force activation, forced expression of MLC(17b) in gizzard smooth muscle cells decreased (p < 0.05) the rate of force activation, while forced expression of the endogenous MLC17 isoform had no effect on the rate of force activation. These results demonstrate that MLC17 is a molecular determinant of the contractile properties of smooth muscle. MLC17 could affect the contractile properties of smooth muscle by either changing the stiffness of the myosin lever arm or modulating the rate of a load-dependent step and/or transition in the actomyosin ATPase cycle.
AB - The molecular determinants of the contractile properties of smooth muscle are poorly understood, and have been suggested to be controlled by splice variant expression of the myosin heavy chain near the 25/50-kDa junction (Kelley, C. A., Takahashi, M., Yu, J. H., and Adelstein, R. S. (1993) J. Biol. Chem. 268, 12848-12854) as well as by differences in the expression of an acidic (MLC(17a)) and a basic (MLC(17b)) isoform of the 17- kDa essential myosin light chain (Nabeshima, Y., Nonomura, Y., and Fujii- Kuriyama, Y. (1987) J. Biol. Chem. 262, 10650810612). To investigate the molecular mechanism that regulates the mechanical properties of smooth muscle, we determined the effect of forced expression of MLC(17a) and MLC(17b) on the rate of force activation during agonist-stimulated contractions of single cultured chicken embryonic aortic and gizzard smooth muscle cells. Forced expression of MLC(17a) in aortic smooth muscle cells increased (p < 0.05) the rate of force activation, forced expression of MLC(17b) in gizzard smooth muscle cells decreased (p < 0.05) the rate of force activation, while forced expression of the endogenous MLC17 isoform had no effect on the rate of force activation. These results demonstrate that MLC17 is a molecular determinant of the contractile properties of smooth muscle. MLC17 could affect the contractile properties of smooth muscle by either changing the stiffness of the myosin lever arm or modulating the rate of a load-dependent step and/or transition in the actomyosin ATPase cycle.
UR - http://www.scopus.com/inward/record.url?scp=0033521035&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033521035&partnerID=8YFLogxK
U2 - 10.1074/jbc.274.49.35095
DO - 10.1074/jbc.274.49.35095
M3 - Article
C2 - 10574990
AN - SCOPUS:0033521035
SN - 0021-9258
VL - 274
SP - 35095
EP - 35098
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 49
ER -