Abstract
The "latch state" or force maintenance may be due to the emergence of a distinct set of dephosphorylated, slowly cycling "latch" cross-bridges, slowing of the overall cross-bridge cycling rate, or a non-cross-bridge contribution. This was investigated by sinusoidally oscillating strips of intact rabbit portal vein or aorta. Tissue strips were activated with KCl depolarization, resulting in a sustained increase of MLC20 phosphorylation or 10μM phenylephrine, resulting in a transient increase in MLC20 phosphorylation. Stiffness was calculated from the force response to a small, sine-wave oscillation in muscle length (1-100Hz). The results produced a 3-dimensional plot of stiffness versus the frequency of oscillation (Hz) versus time (s), or stiffness distribution profile. During KCl depolarization, the stiffness distribution profile displayed a shift toward lower frequencies, suggesting a general slowing in the overall cross-bridge cycling rate during force maintenance. On the other hand, phenylephrine stimulation did not display a significant change in the stiffness distribution profile, suggesting that the overall cross-bridge cycling rate did not significantly change during force maintenance.
Original language | English (US) |
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Pages (from-to) | 25-38 |
Number of pages | 14 |
Journal | Archives of Biochemistry and Biophysics |
Volume | 410 |
Issue number | 1 |
DOIs | |
State | Published - Feb 1 2003 |
Keywords
- Force maintenance
- KCl
- MLC
- Phenylephrine
- Smooth muscle
- Stiffness
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology