Fluorescent-labeled DNA probes applied to novel biological aspects of B-cell chronic lymphocytic leukemia

Stephanie R. Fink, Sarah F. Paternoster, Stephanie A. Smoley, Heather C. Flynn, Susan M. Geyer, Tait D. Shanafelt, You Kyoung Lee, Diane F. Jelinek, Neil E. Kay, Gordon W. Dewald

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Fluorescent-labeled DNA probes were used to study 52 chronic lymphocytic leukemia (B-CLL) patients for (1) disease progression, (2) angiogenesis genes, (3) T-cell leukemia 1 gene (TCL1), (4) immunoglobulin heavy chain variable region (IGHv) and (5) chromosome 6q. Compared to stable disease, more patients with progressive disease had ≥2 anomalies and a high percentage of neoplastic nuclei. Anomalies of genes for basic fibroblast growth factor, interleukin 4, vascular endothelial growth factor or TCL1 were not detected. Deletions in IGHv occurred in 25% of patients and correlated with IGHv gene expression. Probes for 6q23 detected more deletions in 6q than probes for 6q21.

Original languageEnglish (US)
Pages (from-to)253-262
Number of pages10
JournalLeukemia Research
Volume29
Issue number3
DOIs
StatePublished - Mar 1 2005

Keywords

  • Angiogenesis
  • CLL
  • FISH
  • IGHv
  • TCL1

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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