Fluorescent in situ hybridization

Use of whole chromosome paint probes to identify unbalanced chromosome translocations

W. J. Kraker, T. J. Borell, C. R. Schad, M. J. Pennington, P. S. Karnes, G. W. Dewald, Robert Brian Jenkins

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

By identifying structural chromosome anomalies, the clinical cytogenetics laboratory can play a critical role in the diagnosis and treatment of patients with birth defects. Although many new staining techniques have been developed throughout the years to aid in the detection of anomalous chromosomes, some abnormalities still pose a special challenge to cytogeneticists. This difficulty is especially evident in patients with an abnormal chromosome that does not produce a recognizable banding pattern by conventional staining techniques. We describe a recently discovered method of identifying chromosomes by using whole chromosome-specific DNA probes and fluorescent in situ hybridization and provide examples of how this new procedure facilitated the identification of chromosome abnormalities in two patients with multiple birth defects.

Original languageEnglish (US)
Pages (from-to)658-662
Number of pages5
JournalMayo Clinic Proceedings
Volume67
Issue number7
StatePublished - 1992

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Paint
Fluorescence In Situ Hybridization
Chromosomes
Chromosome Aberrations
Staining and Labeling
Multiple Birth Offspring
DNA Probes
Cytogenetics
Therapeutics

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Kraker, W. J., Borell, T. J., Schad, C. R., Pennington, M. J., Karnes, P. S., Dewald, G. W., & Jenkins, R. B. (1992). Fluorescent in situ hybridization: Use of whole chromosome paint probes to identify unbalanced chromosome translocations. Mayo Clinic Proceedings, 67(7), 658-662.

Fluorescent in situ hybridization : Use of whole chromosome paint probes to identify unbalanced chromosome translocations. / Kraker, W. J.; Borell, T. J.; Schad, C. R.; Pennington, M. J.; Karnes, P. S.; Dewald, G. W.; Jenkins, Robert Brian.

In: Mayo Clinic Proceedings, Vol. 67, No. 7, 1992, p. 658-662.

Research output: Contribution to journalArticle

Kraker, WJ, Borell, TJ, Schad, CR, Pennington, MJ, Karnes, PS, Dewald, GW & Jenkins, RB 1992, 'Fluorescent in situ hybridization: Use of whole chromosome paint probes to identify unbalanced chromosome translocations', Mayo Clinic Proceedings, vol. 67, no. 7, pp. 658-662.
Kraker WJ, Borell TJ, Schad CR, Pennington MJ, Karnes PS, Dewald GW et al. Fluorescent in situ hybridization: Use of whole chromosome paint probes to identify unbalanced chromosome translocations. Mayo Clinic Proceedings. 1992;67(7):658-662.
Kraker, W. J. ; Borell, T. J. ; Schad, C. R. ; Pennington, M. J. ; Karnes, P. S. ; Dewald, G. W. ; Jenkins, Robert Brian. / Fluorescent in situ hybridization : Use of whole chromosome paint probes to identify unbalanced chromosome translocations. In: Mayo Clinic Proceedings. 1992 ; Vol. 67, No. 7. pp. 658-662.
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