TY - JOUR
T1 - Fluorescence spectroscopy of endomyocardial tissue post-human heart transplantation
T2 - Does it correlate with histopathology?
AU - Yamani, Mohamad H.
AU - Van De Poll, Sweder W.E.
AU - Ratliff, Norman B.
AU - Kuban, Barry E.
AU - Starling, Randall C.
AU - McCarthy, Patrick M.
AU - Young, James B.
N1 - Funding Information:
The authors gratefully acknowledge Massachusetts Institute of Technology for support of this project. Dr. Sweder W.E. van de Poll received a grant from the Interuniversity Cardiology Institution of the Netherlands (ICIN).
PY - 2000
Y1 - 2000
N2 - Background: A significant correlation between autofluorescence spectroscopy and heart allograft rejection has been described in the rat heterotropic allograft model. However, the use of this technique in human heart transplants has not been validate. Methods: We obtained fluorescence and reflectance spectra on 37 human endomyocardial biopsy specimens and correlated the spectra with International Society Heart and Lung Transplantation grade for histologic rejection. Results: Using different excitation wavelengths (ultraviolet, λ = 337 nm; blue, λ = 440 nm, and green, λ = 486 nm), we found no significant difference in the fluorescence spectra among the different grades of rejection. Conclusions: Fluorescence spectroscopy is not a sensitive method for detecting rejection in human heart transplant recipients.
AB - Background: A significant correlation between autofluorescence spectroscopy and heart allograft rejection has been described in the rat heterotropic allograft model. However, the use of this technique in human heart transplants has not been validate. Methods: We obtained fluorescence and reflectance spectra on 37 human endomyocardial biopsy specimens and correlated the spectra with International Society Heart and Lung Transplantation grade for histologic rejection. Results: Using different excitation wavelengths (ultraviolet, λ = 337 nm; blue, λ = 440 nm, and green, λ = 486 nm), we found no significant difference in the fluorescence spectra among the different grades of rejection. Conclusions: Fluorescence spectroscopy is not a sensitive method for detecting rejection in human heart transplant recipients.
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U2 - 10.1016/S1053-2498(00)00161-3
DO - 10.1016/S1053-2498(00)00161-3
M3 - Article
C2 - 11077225
AN - SCOPUS:0033765594
SN - 1053-2498
VL - 19
SP - 1077
EP - 1080
JO - Journal of Heart and Lung Transplantation
JF - Journal of Heart and Lung Transplantation
IS - 11
ER -