Fluorescence of native and partially denatured variant-3 scorpion neurotoxin

Christopher Haydock; Salah S. Sedarous; Franklyn G. Prendergast

Fluorescence of native and partially denatured variant-3 scorpion neurotoxin

Christopher Haydock, Salah S. Sedarous, Franklyn G. Prendergast

Pharmacology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The multi-component fluorescence intensity decay of variant-3 scorpion neurotoxin is measured with time-correlated single photon counting as a function of guanidine hydrochloride (GuHCl) concentration. Available evidence suggests that while the NH₂-terminal β- sheet strand may be denatured in GuHCl, the remaining core neurotoxin structure remains intact. We investigate this hypothesis with computer simulations of variant-3 scorpion neurotoxin with lysine-1 to tyrosine-4 deleted. Previous combination thermodynamic perturbation and umbrella sampling, adiabatic mapping and minimum perturbation mapping computer simulations of tryptophan-47 in the native neurotoxin exhibited multiple rotational isomers that might correspond to the observed fluorescence intensity decay components. The new simulations allow us to compare the number of rotational isomers, the isomer populations, the order parameters, and the transition state theory isomer interconversion rates in the native and denatured states.

Original language	English (US)
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Publisher	Publ by Int Soc for Optical Engineering
Pages	167-171
Number of pages	5
ISBN (Print)	0819407860
State	Published - Jan 1 1992
Event	Time-Resolved Laser Spectroscopy in Biochemistry III - Los Angeles, CA, USA Duration: Jan 20 1992 → Jan 22 1992

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	1640
ISSN (Print)	0277-786X

Other

Other	Time-Resolved Laser Spectroscopy in Biochemistry III
City	Los Angeles, CA, USA
Period	1/20/92 → 1/22/92

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Cite this

Fluorescence of native and partially denatured variant-3 scorpion neurotoxin. / Haydock, Christopher; Sedarous, Salah S.; Prendergast, Franklyn G.
Proceedings of SPIE - The International Society for Optical Engineering. Publ by Int Soc for Optical Engineering, 1992. p. 167-171 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 1640).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Haydock, C, Sedarous, SS & Prendergast, FG 1992, Fluorescence of native and partially denatured variant-3 scorpion neurotoxin. in Proceedings of SPIE - The International Society for Optical Engineering. Proceedings of SPIE - The International Society for Optical Engineering, vol. 1640, Publ by Int Soc for Optical Engineering, pp. 167-171, Time-Resolved Laser Spectroscopy in Biochemistry III, Los Angeles, CA, USA, 1/20/92.

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AB - The multi-component fluorescence intensity decay of variant-3 scorpion neurotoxin is measured with time-correlated single photon counting as a function of guanidine hydrochloride (GuHCl) concentration. Available evidence suggests that while the NH2-terminal β- sheet strand may be denatured in GuHCl, the remaining core neurotoxin structure remains intact. We investigate this hypothesis with computer simulations of variant-3 scorpion neurotoxin with lysine-1 to tyrosine-4 deleted. Previous combination thermodynamic perturbation and umbrella sampling, adiabatic mapping and minimum perturbation mapping computer simulations of tryptophan-47 in the native neurotoxin exhibited multiple rotational isomers that might correspond to the observed fluorescence intensity decay components. The new simulations allow us to compare the number of rotational isomers, the isomer populations, the order parameters, and the transition state theory isomer interconversion rates in the native and denatured states.

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Fluorescence of native and partially denatured variant-3 scorpion neurotoxin

Abstract

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ASJC Scopus subject areas

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