Fluorescence in situ hybridization analysis of renal oncocytoma reveals frequent loss of chromosomes Y and 1

James A. Brown, Satoru Takahashi, Antonio Alcaraz, Thomas J. Borell, Kari L. Anderl, Junqi Qian, Diane L. Persons, David G. Bostwick, Michael M. Lieber, Robert Brian Jenkins

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Purpose: Cytogenetic studies of a small number of renal oncocytomas have indicated that loss of chromosomes 1 and Y may be involved in the pathogenesis of this tumor. To evaluate these observations further we selected paraffin embedded renal oncocytoma specimens from 20 male and 10 female patients for fluorescence in situ hybridization analysis. Materials and Methods: Isolated nuclei were prepared from paraffin embedded specimens, and fluorescence in situ hybridization was performed with enumeration probes for chromosomes 1, 12, X and Y. Results: Tumors from 10 male (50%) and 4 female (40%) patients demonstrated chromosomal alterations. Loss of chromosome Y was observed in specimens from all 10 male patients, and loss of chromosome 1 or gain of chromosome 12 was noted in 5 and 2 of these specimens, respectively. Of the 4 female patients with chromosomal abnormalities 2 had loss of chromosome 1, 1 had gain of chromosome 1 and 1 had gain of chromosome 12. Conclusions: Our results confirm that loss of chromosomes Y and 1 is common in renal oncocytoma, and that the alterations are probably involved in the pathogenesis of this tumor.

Original languageEnglish (US)
Pages (from-to)31-35
Number of pages5
JournalJournal of Urology
Volume156
Issue number1
DOIs
StatePublished - Jul 1996

Fingerprint

Chromosomes, Human, Pair 1
Fluorescence In Situ Hybridization
Chromosomes, Human, Pair 12
Paraffin
Neoplasms
Y Chromosome
Cytogenetics
Chromosome Aberrations
Renal oncocytoma

Keywords

  • chromosomes
  • in situ hybridization, fluorescence
  • kidney neoplasms

ASJC Scopus subject areas

  • Urology

Cite this

Fluorescence in situ hybridization analysis of renal oncocytoma reveals frequent loss of chromosomes Y and 1. / Brown, James A.; Takahashi, Satoru; Alcaraz, Antonio; Borell, Thomas J.; Anderl, Kari L.; Qian, Junqi; Persons, Diane L.; Bostwick, David G.; Lieber, Michael M.; Jenkins, Robert Brian.

In: Journal of Urology, Vol. 156, No. 1, 07.1996, p. 31-35.

Research output: Contribution to journalArticle

Brown, JA, Takahashi, S, Alcaraz, A, Borell, TJ, Anderl, KL, Qian, J, Persons, DL, Bostwick, DG, Lieber, MM & Jenkins, RB 1996, 'Fluorescence in situ hybridization analysis of renal oncocytoma reveals frequent loss of chromosomes Y and 1', Journal of Urology, vol. 156, no. 1, pp. 31-35. https://doi.org/10.1016/S0022-5347(01)65929-9
Brown, James A. ; Takahashi, Satoru ; Alcaraz, Antonio ; Borell, Thomas J. ; Anderl, Kari L. ; Qian, Junqi ; Persons, Diane L. ; Bostwick, David G. ; Lieber, Michael M. ; Jenkins, Robert Brian. / Fluorescence in situ hybridization analysis of renal oncocytoma reveals frequent loss of chromosomes Y and 1. In: Journal of Urology. 1996 ; Vol. 156, No. 1. pp. 31-35.
@article{0b62ee2a8c024813aabfeb0fd931afe7,
title = "Fluorescence in situ hybridization analysis of renal oncocytoma reveals frequent loss of chromosomes Y and 1",
abstract = "Purpose: Cytogenetic studies of a small number of renal oncocytomas have indicated that loss of chromosomes 1 and Y may be involved in the pathogenesis of this tumor. To evaluate these observations further we selected paraffin embedded renal oncocytoma specimens from 20 male and 10 female patients for fluorescence in situ hybridization analysis. Materials and Methods: Isolated nuclei were prepared from paraffin embedded specimens, and fluorescence in situ hybridization was performed with enumeration probes for chromosomes 1, 12, X and Y. Results: Tumors from 10 male (50{\%}) and 4 female (40{\%}) patients demonstrated chromosomal alterations. Loss of chromosome Y was observed in specimens from all 10 male patients, and loss of chromosome 1 or gain of chromosome 12 was noted in 5 and 2 of these specimens, respectively. Of the 4 female patients with chromosomal abnormalities 2 had loss of chromosome 1, 1 had gain of chromosome 1 and 1 had gain of chromosome 12. Conclusions: Our results confirm that loss of chromosomes Y and 1 is common in renal oncocytoma, and that the alterations are probably involved in the pathogenesis of this tumor.",
keywords = "chromosomes, in situ hybridization, fluorescence, kidney neoplasms",
author = "Brown, {James A.} and Satoru Takahashi and Antonio Alcaraz and Borell, {Thomas J.} and Anderl, {Kari L.} and Junqi Qian and Persons, {Diane L.} and Bostwick, {David G.} and Lieber, {Michael M.} and Jenkins, {Robert Brian}",
year = "1996",
month = "7",
doi = "10.1016/S0022-5347(01)65929-9",
language = "English (US)",
volume = "156",
pages = "31--35",
journal = "Journal of Urology",
issn = "0022-5347",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Fluorescence in situ hybridization analysis of renal oncocytoma reveals frequent loss of chromosomes Y and 1

AU - Brown, James A.

AU - Takahashi, Satoru

AU - Alcaraz, Antonio

AU - Borell, Thomas J.

AU - Anderl, Kari L.

AU - Qian, Junqi

AU - Persons, Diane L.

AU - Bostwick, David G.

AU - Lieber, Michael M.

AU - Jenkins, Robert Brian

PY - 1996/7

Y1 - 1996/7

N2 - Purpose: Cytogenetic studies of a small number of renal oncocytomas have indicated that loss of chromosomes 1 and Y may be involved in the pathogenesis of this tumor. To evaluate these observations further we selected paraffin embedded renal oncocytoma specimens from 20 male and 10 female patients for fluorescence in situ hybridization analysis. Materials and Methods: Isolated nuclei were prepared from paraffin embedded specimens, and fluorescence in situ hybridization was performed with enumeration probes for chromosomes 1, 12, X and Y. Results: Tumors from 10 male (50%) and 4 female (40%) patients demonstrated chromosomal alterations. Loss of chromosome Y was observed in specimens from all 10 male patients, and loss of chromosome 1 or gain of chromosome 12 was noted in 5 and 2 of these specimens, respectively. Of the 4 female patients with chromosomal abnormalities 2 had loss of chromosome 1, 1 had gain of chromosome 1 and 1 had gain of chromosome 12. Conclusions: Our results confirm that loss of chromosomes Y and 1 is common in renal oncocytoma, and that the alterations are probably involved in the pathogenesis of this tumor.

AB - Purpose: Cytogenetic studies of a small number of renal oncocytomas have indicated that loss of chromosomes 1 and Y may be involved in the pathogenesis of this tumor. To evaluate these observations further we selected paraffin embedded renal oncocytoma specimens from 20 male and 10 female patients for fluorescence in situ hybridization analysis. Materials and Methods: Isolated nuclei were prepared from paraffin embedded specimens, and fluorescence in situ hybridization was performed with enumeration probes for chromosomes 1, 12, X and Y. Results: Tumors from 10 male (50%) and 4 female (40%) patients demonstrated chromosomal alterations. Loss of chromosome Y was observed in specimens from all 10 male patients, and loss of chromosome 1 or gain of chromosome 12 was noted in 5 and 2 of these specimens, respectively. Of the 4 female patients with chromosomal abnormalities 2 had loss of chromosome 1, 1 had gain of chromosome 1 and 1 had gain of chromosome 12. Conclusions: Our results confirm that loss of chromosomes Y and 1 is common in renal oncocytoma, and that the alterations are probably involved in the pathogenesis of this tumor.

KW - chromosomes

KW - in situ hybridization, fluorescence

KW - kidney neoplasms

UR - http://www.scopus.com/inward/record.url?scp=8944261602&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=8944261602&partnerID=8YFLogxK

U2 - 10.1016/S0022-5347(01)65929-9

DO - 10.1016/S0022-5347(01)65929-9

M3 - Article

C2 - 8648831

AN - SCOPUS:8944261602

VL - 156

SP - 31

EP - 35

JO - Journal of Urology

JF - Journal of Urology

SN - 0022-5347

IS - 1

ER -