Fluorescence emission and anisotropy from rhodamine dimers

Thomas P. Burghardt, James E. Lyke, Katalin Ajtai

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

The absorption, fluorescence emission, and excitation fluorescence anisotropy spectra of a rhodamine dye in a water-glycerol solution at high concentration were investigated to determine spectroscopic properties of the ground state dimer. The combination of data from these spectra measured at several dye concentrations contained sufficient constraints on the model for dimer association to permit an estimate of the association constant, the extinction coefficients, the relative fluorescence quantum yield, and the emission spectra of the monomeric and dimeric species. The rhodamine dimer is an efficient fluorescence emitter with fluorescence anisotropy equivalent to that of the pure monomeric species over the range of excitation wavelengths covering its two lowest energy transitions.

Original languageEnglish (US)
Pages (from-to)119-131
Number of pages13
JournalBiophysical Chemistry
Volume59
Issue number1-2
DOIs
StatePublished - Mar 7 1996

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Keywords

  • Anisotropy
  • Emission
  • Fluorescence
  • Lifetime
  • Quantum efficiency
  • Rhodamine dimers

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Organic Chemistry

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