Fluid spaces in canine bone and marrow

Michael A. Morris, Jorge A. Lopez-Curto, Sean P F Hughes, Kai Nan An, James B. Bassingthwaighte, Patrick J. Kelly

Research output: Contribution to journalArticle

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Abstract

In order to provide a firm basis for the analysis of experiments on the kinetics of ion and solute exchange in bone, the total water space, extracellular space, and vascular (plasma plus erythrocyte) space in the tibial diaphysis in adult dogs were estimated. Tracers were injected intravenously into 40 dogs after ligation of the renal pedicles. Estimates were made of the volumes of distribution of radioactive tracers of varying characteristics (molecular weight, solubility, and charge), by taking samples of blood, plasma, and bone 3 hr after injection. The volumes of distribution (milliliters per milliliter of cortical bone) were: for tritiated water (a total water marker) 0.245 ± 0.003 (mean ± SD); for [14C]sucrose (an extracellular marker) 0.043 ± 0.001; for 111In-labeled transferrin (a plasma marker) 0.008 ± 0.003; and for red blood cells 0.005 ± 0.002. Interstitial fluid space was estimated by subtracting the estimated plasma space from the estimated extracellular space and it was 0.034 ml/ml of bone. Estimates of haversian canal volumes, obtained morphometrically, were 0.015 ml/ml bone and were compatible with the estimates of the vascular space.

Original languageEnglish (US)
Pages (from-to)188-200
Number of pages13
JournalMicrovascular Research
Volume23
Issue number2
DOIs
StatePublished - 1982

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Canidae
Bone
Bone Marrow
Bone and Bones
Fluids
Extracellular Space
Plasmas
Blood Vessels
Water
Erythrocytes
Haversian System
Dogs
Radioactive Tracers
Blood
Diaphyses
Ion Exchange
Extracellular Fluid
Transferrin
Solubility
Ligation

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine

Cite this

Morris, M. A., Lopez-Curto, J. A., Hughes, S. P. F., An, K. N., Bassingthwaighte, J. B., & Kelly, P. J. (1982). Fluid spaces in canine bone and marrow. Microvascular Research, 23(2), 188-200. https://doi.org/10.1016/0026-2862(82)90064-4

Fluid spaces in canine bone and marrow. / Morris, Michael A.; Lopez-Curto, Jorge A.; Hughes, Sean P F; An, Kai Nan; Bassingthwaighte, James B.; Kelly, Patrick J.

In: Microvascular Research, Vol. 23, No. 2, 1982, p. 188-200.

Research output: Contribution to journalArticle

Morris, MA, Lopez-Curto, JA, Hughes, SPF, An, KN, Bassingthwaighte, JB & Kelly, PJ 1982, 'Fluid spaces in canine bone and marrow', Microvascular Research, vol. 23, no. 2, pp. 188-200. https://doi.org/10.1016/0026-2862(82)90064-4
Morris MA, Lopez-Curto JA, Hughes SPF, An KN, Bassingthwaighte JB, Kelly PJ. Fluid spaces in canine bone and marrow. Microvascular Research. 1982;23(2):188-200. https://doi.org/10.1016/0026-2862(82)90064-4
Morris, Michael A. ; Lopez-Curto, Jorge A. ; Hughes, Sean P F ; An, Kai Nan ; Bassingthwaighte, James B. ; Kelly, Patrick J. / Fluid spaces in canine bone and marrow. In: Microvascular Research. 1982 ; Vol. 23, No. 2. pp. 188-200.
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