TY - JOUR
T1 - Fluid spaces in canine bone and marrow
AU - Morris, Michael A.
AU - Lopez-Curto, Jorge A.
AU - Hughes, Sean P.F.
AU - An, Kai Nan
AU - Bassingthwaighte, James B.
AU - Kelly, Patrick J.
N1 - Funding Information:
’ This investigation was supported in part by Research Grants AM-15980 and HL-19139 from the National Institutes of Health, Public Health Service. M. A. Morris is the recipient of a grant from the Wellcome Foundation. ’ Present address: Stockport Infirmary, Stockport, England. ’ Present address: Mexico City, Mexico. ’ Present address: Princess Margaret Rose Orthopaedic Hospital, Edinburgh, Scotland. ’ Present address: University of Washington, Seattle, Wash. 98195. 6 Reprint requests to: P. J. Kelly, M.D., Mayo Clinic, 200 First Street, S.W., Rochester,‘Minn.
PY - 1982/5
Y1 - 1982/5
N2 - In order to provide a firm basis for the analysis of experiments on the kinetics of ion and solute exchange in bone, the total water space, extracellular space, and vascular (plasma plus erythrocyte) space in the tibial diaphysis in adult dogs were estimated. Tracers were injected intravenously into 40 dogs after ligation of the renal pedicles. Estimates were made of the volumes of distribution of radioactive tracers of varying characteristics (molecular weight, solubility, and charge), by taking samples of blood, plasma, and bone 3 hr after injection. The volumes of distribution (milliliters per milliliter of cortical bone) were: for tritiated water (a total water marker) 0.245 ± 0.003 (mean ± SD); for [14C]sucrose (an extracellular marker) 0.043 ± 0.001; for 111In-labeled transferrin (a plasma marker) 0.008 ± 0.003; and for red blood cells 0.005 ± 0.002. Interstitial fluid space was estimated by subtracting the estimated plasma space from the estimated extracellular space and it was 0.034 ml/ml of bone. Estimates of haversian canal volumes, obtained morphometrically, were 0.015 ml/ml bone and were compatible with the estimates of the vascular space.
AB - In order to provide a firm basis for the analysis of experiments on the kinetics of ion and solute exchange in bone, the total water space, extracellular space, and vascular (plasma plus erythrocyte) space in the tibial diaphysis in adult dogs were estimated. Tracers were injected intravenously into 40 dogs after ligation of the renal pedicles. Estimates were made of the volumes of distribution of radioactive tracers of varying characteristics (molecular weight, solubility, and charge), by taking samples of blood, plasma, and bone 3 hr after injection. The volumes of distribution (milliliters per milliliter of cortical bone) were: for tritiated water (a total water marker) 0.245 ± 0.003 (mean ± SD); for [14C]sucrose (an extracellular marker) 0.043 ± 0.001; for 111In-labeled transferrin (a plasma marker) 0.008 ± 0.003; and for red blood cells 0.005 ± 0.002. Interstitial fluid space was estimated by subtracting the estimated plasma space from the estimated extracellular space and it was 0.034 ml/ml of bone. Estimates of haversian canal volumes, obtained morphometrically, were 0.015 ml/ml bone and were compatible with the estimates of the vascular space.
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U2 - 10.1016/0026-2862(82)90064-4
DO - 10.1016/0026-2862(82)90064-4
M3 - Article
C2 - 7099016
AN - SCOPUS:0020032931
SN - 0026-2862
VL - 23
SP - 188
EP - 200
JO - Microvascular Research
JF - Microvascular Research
IS - 2
ER -