Flow cytometric DNA analysis of early stage adenocarcinoma of the cervix

Paul Magtibay, Jack F. Perrone, C. Robert Stanhope, Jerry A. Katzmann, Gary Keeney, Hongzhe Li

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective. The aim of this study was to determine the utility of DNA flow cytometry as a prognostic indicator for risk of recurrence and overall survival in patients with early stage adenocarcinomas of the uterine cervix. Methods. DNA flow cytometry was performed to determine ploidy, DNA index, and proliferative index in 66 women with stage IB and IIA pure mucinous adenocarcinomas of the cervix treated by primary surgical therapy with radical hysterectomy and pelvic lymphadenectomy. Fifty-seven of 66 (86.3%) tissue samples were analyzable. Three sections were obtained from paraffin- embedded tissue blocks containing primary tumor. Flow cytometric results, along with other known prognostic variables for risk for recurrent disease and survival, were analyzed using the Cox regression proportional hazards model and survival curves generated by the Kaplan-Meier method. Results. Of 57 interpretable samples, DNA ploidy patterns were 18 (27%) diploid, 8 (12%) tetraploid, and 31 (47%) aneuploid. Thirteen of 66 patients (20%) experienced recurrence with a median time to recurrence of 1.6 years. No significant correlation was noted between DNA ploidy and risk of recurrence (P = 0.429). Multivariate analysis confirmed that positive metastatic lymph nodes were associated with risk of recurrence (P < 0.001). In node-negative patients, a high proliferative index (S% + G2M% > 20%), measured as a continuous variable, was the only significant factor for tumor recurrence (P = 0.002). Conclusion. DNA ploidy does not predict a patient's risk for tumor recurrence; however, a high proliferative index value warrants further investigation as a potential prognostic indicator for risk of recurrent disease in patients with adenocarcinoma of the uterine cervix.

Original languageEnglish (US)
Pages (from-to)242-247
Number of pages6
JournalGynecologic Oncology
Volume75
Issue number2
DOIs
StatePublished - 1999

Fingerprint

Cervix Uteri
Adenocarcinoma
Ploidies
Recurrence
DNA
Flow Cytometry
Mucinous Adenocarcinoma
Neoplasms
Tetraploidy
Survival
Kaplan-Meier Estimate
Aneuploidy
Lymph Node Excision
Diploidy
Hysterectomy
Proportional Hazards Models
Paraffin
Multivariate Analysis
Lymph Nodes

Keywords

  • Cervical adenocarcinoma
  • DNA ploidy
  • Flow cytometry
  • Proliferative index

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Oncology

Cite this

Flow cytometric DNA analysis of early stage adenocarcinoma of the cervix. / Magtibay, Paul; Perrone, Jack F.; Stanhope, C. Robert; Katzmann, Jerry A.; Keeney, Gary; Li, Hongzhe.

In: Gynecologic Oncology, Vol. 75, No. 2, 1999, p. 242-247.

Research output: Contribution to journalArticle

Magtibay, Paul ; Perrone, Jack F. ; Stanhope, C. Robert ; Katzmann, Jerry A. ; Keeney, Gary ; Li, Hongzhe. / Flow cytometric DNA analysis of early stage adenocarcinoma of the cervix. In: Gynecologic Oncology. 1999 ; Vol. 75, No. 2. pp. 242-247.
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abstract = "Objective. The aim of this study was to determine the utility of DNA flow cytometry as a prognostic indicator for risk of recurrence and overall survival in patients with early stage adenocarcinomas of the uterine cervix. Methods. DNA flow cytometry was performed to determine ploidy, DNA index, and proliferative index in 66 women with stage IB and IIA pure mucinous adenocarcinomas of the cervix treated by primary surgical therapy with radical hysterectomy and pelvic lymphadenectomy. Fifty-seven of 66 (86.3{\%}) tissue samples were analyzable. Three sections were obtained from paraffin- embedded tissue blocks containing primary tumor. Flow cytometric results, along with other known prognostic variables for risk for recurrent disease and survival, were analyzed using the Cox regression proportional hazards model and survival curves generated by the Kaplan-Meier method. Results. Of 57 interpretable samples, DNA ploidy patterns were 18 (27{\%}) diploid, 8 (12{\%}) tetraploid, and 31 (47{\%}) aneuploid. Thirteen of 66 patients (20{\%}) experienced recurrence with a median time to recurrence of 1.6 years. No significant correlation was noted between DNA ploidy and risk of recurrence (P = 0.429). Multivariate analysis confirmed that positive metastatic lymph nodes were associated with risk of recurrence (P < 0.001). In node-negative patients, a high proliferative index (S{\%} + G2M{\%} > 20{\%}), measured as a continuous variable, was the only significant factor for tumor recurrence (P = 0.002). Conclusion. DNA ploidy does not predict a patient's risk for tumor recurrence; however, a high proliferative index value warrants further investigation as a potential prognostic indicator for risk of recurrent disease in patients with adenocarcinoma of the uterine cervix.",
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N2 - Objective. The aim of this study was to determine the utility of DNA flow cytometry as a prognostic indicator for risk of recurrence and overall survival in patients with early stage adenocarcinomas of the uterine cervix. Methods. DNA flow cytometry was performed to determine ploidy, DNA index, and proliferative index in 66 women with stage IB and IIA pure mucinous adenocarcinomas of the cervix treated by primary surgical therapy with radical hysterectomy and pelvic lymphadenectomy. Fifty-seven of 66 (86.3%) tissue samples were analyzable. Three sections were obtained from paraffin- embedded tissue blocks containing primary tumor. Flow cytometric results, along with other known prognostic variables for risk for recurrent disease and survival, were analyzed using the Cox regression proportional hazards model and survival curves generated by the Kaplan-Meier method. Results. Of 57 interpretable samples, DNA ploidy patterns were 18 (27%) diploid, 8 (12%) tetraploid, and 31 (47%) aneuploid. Thirteen of 66 patients (20%) experienced recurrence with a median time to recurrence of 1.6 years. No significant correlation was noted between DNA ploidy and risk of recurrence (P = 0.429). Multivariate analysis confirmed that positive metastatic lymph nodes were associated with risk of recurrence (P < 0.001). In node-negative patients, a high proliferative index (S% + G2M% > 20%), measured as a continuous variable, was the only significant factor for tumor recurrence (P = 0.002). Conclusion. DNA ploidy does not predict a patient's risk for tumor recurrence; however, a high proliferative index value warrants further investigation as a potential prognostic indicator for risk of recurrent disease in patients with adenocarcinoma of the uterine cervix.

AB - Objective. The aim of this study was to determine the utility of DNA flow cytometry as a prognostic indicator for risk of recurrence and overall survival in patients with early stage adenocarcinomas of the uterine cervix. Methods. DNA flow cytometry was performed to determine ploidy, DNA index, and proliferative index in 66 women with stage IB and IIA pure mucinous adenocarcinomas of the cervix treated by primary surgical therapy with radical hysterectomy and pelvic lymphadenectomy. Fifty-seven of 66 (86.3%) tissue samples were analyzable. Three sections were obtained from paraffin- embedded tissue blocks containing primary tumor. Flow cytometric results, along with other known prognostic variables for risk for recurrent disease and survival, were analyzed using the Cox regression proportional hazards model and survival curves generated by the Kaplan-Meier method. Results. Of 57 interpretable samples, DNA ploidy patterns were 18 (27%) diploid, 8 (12%) tetraploid, and 31 (47%) aneuploid. Thirteen of 66 patients (20%) experienced recurrence with a median time to recurrence of 1.6 years. No significant correlation was noted between DNA ploidy and risk of recurrence (P = 0.429). Multivariate analysis confirmed that positive metastatic lymph nodes were associated with risk of recurrence (P < 0.001). In node-negative patients, a high proliferative index (S% + G2M% > 20%), measured as a continuous variable, was the only significant factor for tumor recurrence (P = 0.002). Conclusion. DNA ploidy does not predict a patient's risk for tumor recurrence; however, a high proliferative index value warrants further investigation as a potential prognostic indicator for risk of recurrent disease in patients with adenocarcinoma of the uterine cervix.

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