Flow cytometric analysis of surface light chain expression patterns in B-cell lymphomas using monoclonal and polyclonal antibodies

Pedro Horna, Horatiu Olteanu, Steven H. Kroft, Alexandra M. Harrington

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Light chain (LC) expression by flow cytometry (FC) in B cell non-Hodgkin lymphomas (B-NHLs) can occasionally be detected with one anti-LC antibody but not with another. We retrospectively analyzed 564 four-color FC files from B-NHLs, assessing LC staining with monoclonal antibodies (mAbs) and polyclonal antibodies (pAbs). Discrepancies in LC expression between mAbs and pAbs were present in 9.2% of cases, mainly in chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL; 11.1%), diffuse large B-cell lymphoma (DLBCL; 10.2%), follicular lymphoma (9.5%), and mantle cell lymphoma (11.1%) and most frequently in body fluids. Equal proportions of cases were LC+ only with pAbs (4.8%) or mAbs (4.4%). Negative LC expression with both antibodies was present in 7.5% of cases, most frequently in DLBCL (21.6%) and body fluids (27.6%). Evaluation with both mAbs and pAbs increases the sensitivity for LC detection, with no single reagent outperforming the other, although CLL/SLL preferentially showed LC expression with pAbs.

Original languageEnglish (US)
Pages (from-to)954-959
Number of pages6
JournalAmerican journal of clinical pathology
Volume136
Issue number6
DOIs
StatePublished - Dec 2011

Keywords

  • B-cell lymphomas
  • Flow cytometry
  • Immunoglobulin light chains

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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