Flow cytometric analysis of cytologic specimens in hematologic disease

C. A. Hanson, B. Schnitzer

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cytologic evaluation of body fluids and fine needle aspirations (FNA) is frequently required in patients with hematologic diseases. In this study we have correlated immunophenotyping and DNA analysis by flow cytometry with cytologic findings and tissue biopsies from 20 patients with body fluid specimens and 5 with FNA. Nineteen of 25 cases, including all FNA cases, had an immunophenotype consistent with malignancy: 12 monoclonal B-cell lymphomas, 2 T-cell lymphomas, 2 T-ALL and 3 non-T-ALL. By cytologic examination, 14 of these 19 cases were positive for malignant cells, 2 suspicious and 3 negative; the latter 5 cases, including 2 FNA cases, had small monoclonal B-cell populations detected by flow cytometry. Six cases had a benign immunophenotype; cytologic examination was benign in 4 of these and suspicious for lymphoma in 2. Our results show the feasibility of using flow cytometry to evaluate body fluids or FNA and demonstrate that small malignant populations that may be missed by routine cytology can be detected by flow cytometry.

Original languageEnglish (US)
Pages (from-to)2-7
Number of pages6
JournalJournal of Clinical Laboratory Analysis
Volume3
Issue number1
DOIs
StatePublished - 1989
Externally publishedYes

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Hematologic Diseases
Fine Needle Biopsy
Needles
Flow cytometry
Body fluids
Flow Cytometry
Body Fluids
Cells
Cytology
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
Immunophenotyping
T-cells
Biopsy
T-Cell Lymphoma
B-Cell Lymphoma
Population
Cell Biology
Lymphoma
B-Lymphocytes
Tissue

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Flow cytometric analysis of cytologic specimens in hematologic disease. / Hanson, C. A.; Schnitzer, B.

In: Journal of Clinical Laboratory Analysis, Vol. 3, No. 1, 1989, p. 2-7.

Research output: Contribution to journalArticle

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