TY - JOUR
T1 - Fitness of cell-mediated immunity independent of repertoire diversity
AU - AbuAttieh, Mouhammed
AU - Rebrovich, Michelle
AU - Wettstein, Peter J.
AU - Vuk-Pavlovic, Zvezdana
AU - Limper, Andrew H.
AU - Platt, Jeffrey L.
AU - Cascalho, Marilia
PY - 2007/3/1
Y1 - 2007/3/1
N2 - Fitness of cell-mediated immunity is thought to depend on TCR diversity; however, this concept has not been tested formally. We tested the concept using JH-/- mice that lack B cells and have TCR Vβ diversity <1% that of wild-type mice and quasi-monoclonal (QM) mice with oligoclonal B cells and TCR Vβ diversity 7% that of wild-type mice. Despite having a TCR repertoire contracted >99% and defective lymphoid organogenesis, JH -/- mice rejected H-Y-incompatible skin grafts as rapidly as wild-type mice. JH-/- mice exhibited T cell priming by peptide and delayed-type hypersensitivity, although these responses were less than normal owing either to TCR repertoire contraction or defective lymphoid organogenesis. QM mice with TCR diversity contracted >90%, and normal lymphoid organs rejected H-Y incompatible skin grafts as rapidly as wild type mice and exhibited normal T cell priming and normal delayed-type hypersensitivity reactions. QM mice also resisted Pneumocystis murina like wild-type mice. Thus, cell-mediated immunity can function normally despite contractions of TCR diversity >90% and possibly >99%.
AB - Fitness of cell-mediated immunity is thought to depend on TCR diversity; however, this concept has not been tested formally. We tested the concept using JH-/- mice that lack B cells and have TCR Vβ diversity <1% that of wild-type mice and quasi-monoclonal (QM) mice with oligoclonal B cells and TCR Vβ diversity 7% that of wild-type mice. Despite having a TCR repertoire contracted >99% and defective lymphoid organogenesis, JH -/- mice rejected H-Y-incompatible skin grafts as rapidly as wild-type mice. JH-/- mice exhibited T cell priming by peptide and delayed-type hypersensitivity, although these responses were less than normal owing either to TCR repertoire contraction or defective lymphoid organogenesis. QM mice with TCR diversity contracted >90%, and normal lymphoid organs rejected H-Y incompatible skin grafts as rapidly as wild type mice and exhibited normal T cell priming and normal delayed-type hypersensitivity reactions. QM mice also resisted Pneumocystis murina like wild-type mice. Thus, cell-mediated immunity can function normally despite contractions of TCR diversity >90% and possibly >99%.
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U2 - 10.4049/jimmunol.178.5.2950
DO - 10.4049/jimmunol.178.5.2950
M3 - Article
C2 - 17312140
AN - SCOPUS:33847356373
SN - 0022-1767
VL - 178
SP - 2950
EP - 2960
JO - Journal of Immunology
JF - Journal of Immunology
IS - 5
ER -