Abstract
Detection of t(14;18)(q32;q21)-IgH/bcl-2, which is present in 70% to 95% of follicular lymphomas (FLs), might aid in diagnosing FL The efficacy of routine polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) techniques in detecting t(14;18) in paraffin-embedded tissue samples was compared on 5 normal tonsils and 28 FLs demonstrated to be t(14;18)+ by previous karyotyping. There was technical failure in 14 (50%) of the FLs by PCR, likely due to B-5 fixation, and 4 (14%) of FLs by FISH, likely due to advanced specimen age. In the remaining successful cases, 5 (36%) of 14 were positive by PCR and 24 (100%) of 24 were positive by FISH. All 5 normal tonsils were negative by both methods. FISH is superior to PCR for detecting t(14;18) from paraffin-embedded tissue samples because it is more sensitive and equally specific.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 421-429 |
| Number of pages | 9 |
| Journal | American journal of clinical pathology |
| Volume | 124 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 2005 |
Keywords
- FISH
- Fluorescence in situ hybridization
- Follicular lymphoma
- IgH/bcl-2
- PCR
- Paraffin-embedded tissue
- Polymerase chain reaction
- t(14;18)(q32;q21)
ASJC Scopus subject areas
- Pathology and Forensic Medicine