First exons and introns - A survey of GC content and gene structure in the human genome

Krishna R Kalari, Melanie Casavant, Thomas B. Bair, Henry L. Keen, Josep M. Comeron, Thomas L. Casavant, Todd E. Scheetz

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Most transcriptional regulatory elements are located in non-coding DNA. In particular, some first introns play a vital role in transcriptional control and splicing. The length and GC-content of first exons and introns in complex organisms suggests that these structural units are likely to be important functional elements in large genomes. Hence, in this paper we perform a systematic comparison of exon-intron structure and GC content on all known genes in the human genome. Our in-silico analysis found that the GC content of introns and exons varies significantly depending on their length. On average, the first intron of a gene is significantly longer than other introns in the same gene. Our results also show that first introns and exons are more GC rich than last and internal. This study provides insight into the structure of eukaryotic genes. These results confirm and expand the previously identified regulatory potential of first exons and introns.

Original languageEnglish (US)
Pages (from-to)237-242
Number of pages6
JournalIn Silico Biology
Volume6
Issue number3
StatePublished - 2006
Externally publishedYes

Fingerprint

Base Composition
Human Genome
Introns
Exons
Genome
Genes
Gene
Expand
Transcriptional Regulatory Elements
Likely
Vary
Internal
Unit
Surveys and Questionnaires
Human
DNA
Computer Simulation

Keywords

  • GC-content
  • Gene structure
  • Intron length

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Kalari, K. R., Casavant, M., Bair, T. B., Keen, H. L., Comeron, J. M., Casavant, T. L., & Scheetz, T. E. (2006). First exons and introns - A survey of GC content and gene structure in the human genome. In Silico Biology, 6(3), 237-242.

First exons and introns - A survey of GC content and gene structure in the human genome. / Kalari, Krishna R; Casavant, Melanie; Bair, Thomas B.; Keen, Henry L.; Comeron, Josep M.; Casavant, Thomas L.; Scheetz, Todd E.

In: In Silico Biology, Vol. 6, No. 3, 2006, p. 237-242.

Research output: Contribution to journalArticle

Kalari, KR, Casavant, M, Bair, TB, Keen, HL, Comeron, JM, Casavant, TL & Scheetz, TE 2006, 'First exons and introns - A survey of GC content and gene structure in the human genome', In Silico Biology, vol. 6, no. 3, pp. 237-242.
Kalari KR, Casavant M, Bair TB, Keen HL, Comeron JM, Casavant TL et al. First exons and introns - A survey of GC content and gene structure in the human genome. In Silico Biology. 2006;6(3):237-242.
Kalari, Krishna R ; Casavant, Melanie ; Bair, Thomas B. ; Keen, Henry L. ; Comeron, Josep M. ; Casavant, Thomas L. ; Scheetz, Todd E. / First exons and introns - A survey of GC content and gene structure in the human genome. In: In Silico Biology. 2006 ; Vol. 6, No. 3. pp. 237-242.
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