FilmArray respiratory panel assay: Comparison of nasopharyngeal swabs and bronchoalveolar lavage samples

Natalya Azadeh, Kenneth K. Sakata, Anjuli M. Brighton, Holenarasipur R. Vikram, Thomas Grys

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The FilmArray respiratory panel (FARP) reliably and rapidly identifies 17 viruses and 3 bacterial pathogens. A nasopharyngeal swab FARP (NP FARP) is performed for many patients with respiratory symptoms. For patients who are acutely ill or immunocompromised or fail to improve, a bronchoalveolar lavage sample FARP (BAL FARP) is performed in addition to the NP FARP. To date, no studies have compared the yield of a BAL FARP with that of an NP FARP. We retrospectively studied all patients who had a BAL FARP within 7 days after an NP FARP between June 2013 and May 2014. Demographic information, comorbidities, FARP results, and all microbiologic data from BAL fluid were collected. Eighty-six patients had a BAL FARP performed within 7 days (mean, 1.6; median, 1) after an NP FARP. Of these, 66 (77%) had concordant BAL and NP FARP results: 15 (23%) had the same pathogen identified from the NP and BAL FARPs, and 51 (77%) had concordant negative FARP results. In 18 of the 86 patients (21%), a pathogen was detected from the NP FARP; of these, 15 (83%) had a concordant match on a subsequent BAL FARP, and the remaining 3 had negative BAL FARPs. In 17 of the 86 patients (20%), pathogens were identified from the BAL FARPs that were not detected by the NP FARPs; of these, 16 (94%) had initial negative NP FARPs. The data suggest that once a pathogen is identified by an NP FARP, a subsequent BAL FARP is unlikely to add new microbiologic information. However, a BAL FARP may provide new, useful microbiologic information when performed within 7 days after a negative NP FARP.

Original languageEnglish (US)
Pages (from-to)3784-3787
Number of pages4
JournalJournal of Clinical Microbiology
Volume53
Issue number12
DOIs
StatePublished - Dec 1 2015

Fingerprint

Bronchoalveolar Lavage
Dimercaprol
Comorbidity
Demography
Viruses

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

FilmArray respiratory panel assay : Comparison of nasopharyngeal swabs and bronchoalveolar lavage samples. / Azadeh, Natalya; Sakata, Kenneth K.; Brighton, Anjuli M.; Vikram, Holenarasipur R.; Grys, Thomas.

In: Journal of Clinical Microbiology, Vol. 53, No. 12, 01.12.2015, p. 3784-3787.

Research output: Contribution to journalArticle

Azadeh, Natalya ; Sakata, Kenneth K. ; Brighton, Anjuli M. ; Vikram, Holenarasipur R. ; Grys, Thomas. / FilmArray respiratory panel assay : Comparison of nasopharyngeal swabs and bronchoalveolar lavage samples. In: Journal of Clinical Microbiology. 2015 ; Vol. 53, No. 12. pp. 3784-3787.
@article{b98515321f384da4bb0fb26709711df9,
title = "FilmArray respiratory panel assay: Comparison of nasopharyngeal swabs and bronchoalveolar lavage samples",
abstract = "The FilmArray respiratory panel (FARP) reliably and rapidly identifies 17 viruses and 3 bacterial pathogens. A nasopharyngeal swab FARP (NP FARP) is performed for many patients with respiratory symptoms. For patients who are acutely ill or immunocompromised or fail to improve, a bronchoalveolar lavage sample FARP (BAL FARP) is performed in addition to the NP FARP. To date, no studies have compared the yield of a BAL FARP with that of an NP FARP. We retrospectively studied all patients who had a BAL FARP within 7 days after an NP FARP between June 2013 and May 2014. Demographic information, comorbidities, FARP results, and all microbiologic data from BAL fluid were collected. Eighty-six patients had a BAL FARP performed within 7 days (mean, 1.6; median, 1) after an NP FARP. Of these, 66 (77{\%}) had concordant BAL and NP FARP results: 15 (23{\%}) had the same pathogen identified from the NP and BAL FARPs, and 51 (77{\%}) had concordant negative FARP results. In 18 of the 86 patients (21{\%}), a pathogen was detected from the NP FARP; of these, 15 (83{\%}) had a concordant match on a subsequent BAL FARP, and the remaining 3 had negative BAL FARPs. In 17 of the 86 patients (20{\%}), pathogens were identified from the BAL FARPs that were not detected by the NP FARPs; of these, 16 (94{\%}) had initial negative NP FARPs. The data suggest that once a pathogen is identified by an NP FARP, a subsequent BAL FARP is unlikely to add new microbiologic information. However, a BAL FARP may provide new, useful microbiologic information when performed within 7 days after a negative NP FARP.",
author = "Natalya Azadeh and Sakata, {Kenneth K.} and Brighton, {Anjuli M.} and Vikram, {Holenarasipur R.} and Thomas Grys",
year = "2015",
month = "12",
day = "1",
doi = "10.1128/JCM.01516-15",
language = "English (US)",
volume = "53",
pages = "3784--3787",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "12",

}

TY - JOUR

T1 - FilmArray respiratory panel assay

T2 - Comparison of nasopharyngeal swabs and bronchoalveolar lavage samples

AU - Azadeh, Natalya

AU - Sakata, Kenneth K.

AU - Brighton, Anjuli M.

AU - Vikram, Holenarasipur R.

AU - Grys, Thomas

PY - 2015/12/1

Y1 - 2015/12/1

N2 - The FilmArray respiratory panel (FARP) reliably and rapidly identifies 17 viruses and 3 bacterial pathogens. A nasopharyngeal swab FARP (NP FARP) is performed for many patients with respiratory symptoms. For patients who are acutely ill or immunocompromised or fail to improve, a bronchoalveolar lavage sample FARP (BAL FARP) is performed in addition to the NP FARP. To date, no studies have compared the yield of a BAL FARP with that of an NP FARP. We retrospectively studied all patients who had a BAL FARP within 7 days after an NP FARP between June 2013 and May 2014. Demographic information, comorbidities, FARP results, and all microbiologic data from BAL fluid were collected. Eighty-six patients had a BAL FARP performed within 7 days (mean, 1.6; median, 1) after an NP FARP. Of these, 66 (77%) had concordant BAL and NP FARP results: 15 (23%) had the same pathogen identified from the NP and BAL FARPs, and 51 (77%) had concordant negative FARP results. In 18 of the 86 patients (21%), a pathogen was detected from the NP FARP; of these, 15 (83%) had a concordant match on a subsequent BAL FARP, and the remaining 3 had negative BAL FARPs. In 17 of the 86 patients (20%), pathogens were identified from the BAL FARPs that were not detected by the NP FARPs; of these, 16 (94%) had initial negative NP FARPs. The data suggest that once a pathogen is identified by an NP FARP, a subsequent BAL FARP is unlikely to add new microbiologic information. However, a BAL FARP may provide new, useful microbiologic information when performed within 7 days after a negative NP FARP.

AB - The FilmArray respiratory panel (FARP) reliably and rapidly identifies 17 viruses and 3 bacterial pathogens. A nasopharyngeal swab FARP (NP FARP) is performed for many patients with respiratory symptoms. For patients who are acutely ill or immunocompromised or fail to improve, a bronchoalveolar lavage sample FARP (BAL FARP) is performed in addition to the NP FARP. To date, no studies have compared the yield of a BAL FARP with that of an NP FARP. We retrospectively studied all patients who had a BAL FARP within 7 days after an NP FARP between June 2013 and May 2014. Demographic information, comorbidities, FARP results, and all microbiologic data from BAL fluid were collected. Eighty-six patients had a BAL FARP performed within 7 days (mean, 1.6; median, 1) after an NP FARP. Of these, 66 (77%) had concordant BAL and NP FARP results: 15 (23%) had the same pathogen identified from the NP and BAL FARPs, and 51 (77%) had concordant negative FARP results. In 18 of the 86 patients (21%), a pathogen was detected from the NP FARP; of these, 15 (83%) had a concordant match on a subsequent BAL FARP, and the remaining 3 had negative BAL FARPs. In 17 of the 86 patients (20%), pathogens were identified from the BAL FARPs that were not detected by the NP FARPs; of these, 16 (94%) had initial negative NP FARPs. The data suggest that once a pathogen is identified by an NP FARP, a subsequent BAL FARP is unlikely to add new microbiologic information. However, a BAL FARP may provide new, useful microbiologic information when performed within 7 days after a negative NP FARP.

UR - http://www.scopus.com/inward/record.url?scp=84947800959&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84947800959&partnerID=8YFLogxK

U2 - 10.1128/JCM.01516-15

DO - 10.1128/JCM.01516-15

M3 - Article

C2 - 26378282

AN - SCOPUS:84947800959

VL - 53

SP - 3784

EP - 3787

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 12

ER -