Fibronectin enhances in vitro monocyte-macrophage-mediated tumoricidal activity

R. T. Perri, Neil Elliot Kay, J. McCarthy, R. L. Vessella, H. S. Jacob, L. T. Furcht

Research output: Contribution to journalArticle

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Abstract

Control of neoplastic proliferation reflects in part monocyte/macrophage destruction of target cells - destruction that evidently requires cell-cell interaction. We herein show it to involve the natural plasma opsonin, fibronectin. With two cultured human tumor lines - Malme melanoma and CAK-I renal carcinoma cells - addition of fibronectin, purified to homogeneity, enhances macrophage-mediated cytotoxicity 2-4 fold (p<0.01). Both fresh human monocytes or the U-937-cultured macrophage line become more lethal to tumor cells with added fibronectin. The fibronectin-enhanced monocyte and U-937 tumoricidal activity occurred in a dose-dependent fashion. Specificity of fibronectin's action was validated by use of affinity-purified rabbit, antifibronectin antibody, which completely abated its enhancement of tumoricidal activity. Enhancement of tumoricidal activity did not occur when monocytes or U-937 were exposed to fibronectin-coated plates. However, the addition of soluble fibronectin-coated plates was then capable of enhancing cytocidal activity. These studies demonstrate that human fibronectin is capable of increasing both fresh and cultured human monocyte cumor-directed cytotoxicity. Fibronectin appears to be a potentially important circulating molecule that may favorably influence human monocyte tumor cell cytotoxicity.

Original languageEnglish (US)
Pages (from-to)430-435
Number of pages6
JournalBlood
Volume60
Issue number2
StatePublished - 1982
Externally publishedYes

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Macrophages
Fibronectins
Monocytes
Cytotoxicity
Tumors
Cells
Opsonin Proteins
In Vitro Techniques
Neoplasms
Renal Cell Carcinoma
Cell Communication
Melanoma
Rabbits
Plasmas
Molecules
Antibodies

ASJC Scopus subject areas

  • Hematology

Cite this

Perri, R. T., Kay, N. E., McCarthy, J., Vessella, R. L., Jacob, H. S., & Furcht, L. T. (1982). Fibronectin enhances in vitro monocyte-macrophage-mediated tumoricidal activity. Blood, 60(2), 430-435.

Fibronectin enhances in vitro monocyte-macrophage-mediated tumoricidal activity. / Perri, R. T.; Kay, Neil Elliot; McCarthy, J.; Vessella, R. L.; Jacob, H. S.; Furcht, L. T.

In: Blood, Vol. 60, No. 2, 1982, p. 430-435.

Research output: Contribution to journalArticle

Perri, RT, Kay, NE, McCarthy, J, Vessella, RL, Jacob, HS & Furcht, LT 1982, 'Fibronectin enhances in vitro monocyte-macrophage-mediated tumoricidal activity', Blood, vol. 60, no. 2, pp. 430-435.
Perri RT, Kay NE, McCarthy J, Vessella RL, Jacob HS, Furcht LT. Fibronectin enhances in vitro monocyte-macrophage-mediated tumoricidal activity. Blood. 1982;60(2):430-435.
Perri, R. T. ; Kay, Neil Elliot ; McCarthy, J. ; Vessella, R. L. ; Jacob, H. S. ; Furcht, L. T. / Fibronectin enhances in vitro monocyte-macrophage-mediated tumoricidal activity. In: Blood. 1982 ; Vol. 60, No. 2. pp. 430-435.
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