Fibroblast growth factor-2 and vascular endothelial growth factor mediated augmentation of angiogenesis and bone formation in vascularized bone allotransplants

Mikko Larsen, Wouter F. Willems, Michael Pelzer, Patricia F. Friedrich, Mahrokh Dadsetan, Allen Thorp Bishop

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We previously demonstrated recipient-derived neoangiogenesis to maintain viability of living bone allogeneic transplants without long-term immunosuppression. The effect of cytokine delivery to enhance this process is studied. Vascularized femur transplantation was performed from Dark Agouti to Piebald Virol Glaxo rats. Poly(d,l-lactide-co-glycolide) microspheres loaded with buffer (N = 11), basic fibroblast growth factor (FGF2) (N = 10), vascular endothelial growth factor (VEGF) (N = 11), or both (N = 11) were inserted intramedullarly alongside a recipient-derived arteriovenous bundle. FK-506 was administered for 2 weeks. At 18 weeks, bone blood flow, microangiography, histologic, histomorphometric, and alkaline phosphatase measurements were performed. Bone blood flow was greater in the combined group than control and VEGF groups (P = 0.04). Capillary density was greater in the FGF2 group than in the VEGF and combined groups (P < 0.05). Bone viability, growth, and alkaline phosphatase activity did not vary significantly between groups. Neoangiogenesis in vascularized bone allotransplants is enhanced by angiogenic cytokine delivery, with results using FGF2 that are comparable to isotransplant from previous studies. Further studies are needed to achieve bone formation similar to isotransplants.

Original languageEnglish (US)
Pages (from-to)301-307
Number of pages7
JournalMicrosurgery
Volume34
Issue number4
DOIs
StatePublished - 2014

Fingerprint

Fibroblast Growth Factor 2
Osteogenesis
Vascular Endothelial Growth Factor A
Isografts
Bone and Bones
Alkaline Phosphatase
Cytokines
Bone Development
Tacrolimus
Microspheres
Femur
Immunosuppression
Buffers
Transplantation
Transplants
Control Groups

ASJC Scopus subject areas

  • Surgery
  • Medicine(all)

Cite this

Fibroblast growth factor-2 and vascular endothelial growth factor mediated augmentation of angiogenesis and bone formation in vascularized bone allotransplants. / Larsen, Mikko; Willems, Wouter F.; Pelzer, Michael; Friedrich, Patricia F.; Dadsetan, Mahrokh; Bishop, Allen Thorp.

In: Microsurgery, Vol. 34, No. 4, 2014, p. 301-307.

Research output: Contribution to journalArticle

Larsen, Mikko ; Willems, Wouter F. ; Pelzer, Michael ; Friedrich, Patricia F. ; Dadsetan, Mahrokh ; Bishop, Allen Thorp. / Fibroblast growth factor-2 and vascular endothelial growth factor mediated augmentation of angiogenesis and bone formation in vascularized bone allotransplants. In: Microsurgery. 2014 ; Vol. 34, No. 4. pp. 301-307.
@article{bb9bd17ec41c4dbb8f65a1513f5fcd7a,
title = "Fibroblast growth factor-2 and vascular endothelial growth factor mediated augmentation of angiogenesis and bone formation in vascularized bone allotransplants",
abstract = "We previously demonstrated recipient-derived neoangiogenesis to maintain viability of living bone allogeneic transplants without long-term immunosuppression. The effect of cytokine delivery to enhance this process is studied. Vascularized femur transplantation was performed from Dark Agouti to Piebald Virol Glaxo rats. Poly(d,l-lactide-co-glycolide) microspheres loaded with buffer (N = 11), basic fibroblast growth factor (FGF2) (N = 10), vascular endothelial growth factor (VEGF) (N = 11), or both (N = 11) were inserted intramedullarly alongside a recipient-derived arteriovenous bundle. FK-506 was administered for 2 weeks. At 18 weeks, bone blood flow, microangiography, histologic, histomorphometric, and alkaline phosphatase measurements were performed. Bone blood flow was greater in the combined group than control and VEGF groups (P = 0.04). Capillary density was greater in the FGF2 group than in the VEGF and combined groups (P < 0.05). Bone viability, growth, and alkaline phosphatase activity did not vary significantly between groups. Neoangiogenesis in vascularized bone allotransplants is enhanced by angiogenic cytokine delivery, with results using FGF2 that are comparable to isotransplant from previous studies. Further studies are needed to achieve bone formation similar to isotransplants.",
author = "Mikko Larsen and Willems, {Wouter F.} and Michael Pelzer and Friedrich, {Patricia F.} and Mahrokh Dadsetan and Bishop, {Allen Thorp}",
year = "2014",
doi = "10.1002/micr.22221",
language = "English (US)",
volume = "34",
pages = "301--307",
journal = "Microsurgery",
issn = "0738-1085",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Fibroblast growth factor-2 and vascular endothelial growth factor mediated augmentation of angiogenesis and bone formation in vascularized bone allotransplants

AU - Larsen, Mikko

AU - Willems, Wouter F.

AU - Pelzer, Michael

AU - Friedrich, Patricia F.

AU - Dadsetan, Mahrokh

AU - Bishop, Allen Thorp

PY - 2014

Y1 - 2014

N2 - We previously demonstrated recipient-derived neoangiogenesis to maintain viability of living bone allogeneic transplants without long-term immunosuppression. The effect of cytokine delivery to enhance this process is studied. Vascularized femur transplantation was performed from Dark Agouti to Piebald Virol Glaxo rats. Poly(d,l-lactide-co-glycolide) microspheres loaded with buffer (N = 11), basic fibroblast growth factor (FGF2) (N = 10), vascular endothelial growth factor (VEGF) (N = 11), or both (N = 11) were inserted intramedullarly alongside a recipient-derived arteriovenous bundle. FK-506 was administered for 2 weeks. At 18 weeks, bone blood flow, microangiography, histologic, histomorphometric, and alkaline phosphatase measurements were performed. Bone blood flow was greater in the combined group than control and VEGF groups (P = 0.04). Capillary density was greater in the FGF2 group than in the VEGF and combined groups (P < 0.05). Bone viability, growth, and alkaline phosphatase activity did not vary significantly between groups. Neoangiogenesis in vascularized bone allotransplants is enhanced by angiogenic cytokine delivery, with results using FGF2 that are comparable to isotransplant from previous studies. Further studies are needed to achieve bone formation similar to isotransplants.

AB - We previously demonstrated recipient-derived neoangiogenesis to maintain viability of living bone allogeneic transplants without long-term immunosuppression. The effect of cytokine delivery to enhance this process is studied. Vascularized femur transplantation was performed from Dark Agouti to Piebald Virol Glaxo rats. Poly(d,l-lactide-co-glycolide) microspheres loaded with buffer (N = 11), basic fibroblast growth factor (FGF2) (N = 10), vascular endothelial growth factor (VEGF) (N = 11), or both (N = 11) were inserted intramedullarly alongside a recipient-derived arteriovenous bundle. FK-506 was administered for 2 weeks. At 18 weeks, bone blood flow, microangiography, histologic, histomorphometric, and alkaline phosphatase measurements were performed. Bone blood flow was greater in the combined group than control and VEGF groups (P = 0.04). Capillary density was greater in the FGF2 group than in the VEGF and combined groups (P < 0.05). Bone viability, growth, and alkaline phosphatase activity did not vary significantly between groups. Neoangiogenesis in vascularized bone allotransplants is enhanced by angiogenic cytokine delivery, with results using FGF2 that are comparable to isotransplant from previous studies. Further studies are needed to achieve bone formation similar to isotransplants.

UR - http://www.scopus.com/inward/record.url?scp=84897991533&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84897991533&partnerID=8YFLogxK

U2 - 10.1002/micr.22221

DO - 10.1002/micr.22221

M3 - Article

VL - 34

SP - 301

EP - 307

JO - Microsurgery

JF - Microsurgery

SN - 0738-1085

IS - 4

ER -