Fetal human airway smooth muscle cell production of leukocyte chemoattractants is differentially regulated by fluticasone

Helen Pearson, Rodney Jr. Britt, Christina M Pabelick, Y.s. Prakash, Yassine Amrani, Hitesh C. Pandya

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Adult human airway smooth muscle (ASM) produce cytokines involved in recruitment and survival of leukocytes within airway walls. Cytokine generation by adult ASM is glucocorticoid-sensitive. Whether developing lung ASM produces cytokines in a glucocorticoid-sensitive fashion is unknown.Methods:Cultured fetal human ASM cells stimulated with TNF-α (0-20 ng/ml) were incubated with TNF-α receptor-blocking antibodies, fluticasone (1 and 100 nm), or vehicle. Supernatants and cells were assayed for the production of CCL5, CXCL10, and CXCL8 mRNA and protein and glucocorticoid receptor phosphorylation.Results:CCL5, CXCL10, and CXCL8 mRNA and protein production by fetal ASM cell was significantly and dose-dependently following TNF-α treatment. Cytokine mRNA and protein production were effectively blocked by TNF-α R1 and R2 receptor neutralizing antibodies but variably inhibited by fluticasone. TNF-α-induced TNF-R1 and R2 receptor mRNA expression was only partially attenuated by fluticasone. Glucocorticoid receptor phosphorylation at serine (Ser) 211 but not at Ser 226 was enhanced by fluticasone.Conclusion:Production of CCL5, CXCL10, and CXCL8 by fetal ASM appears to involve pathways that are both qualitatively and mechanistically distinct to those described for adult ASM. The findings imply developing ASM has potential to recruit leukocyte into airways and, therefore, of relevance to childhood airway diseases.

Original languageEnglish (US)
Pages (from-to)650-656
Number of pages7
JournalPediatric Research
Volume78
Issue number6
DOIs
StatePublished - Dec 1 2015

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Chemotactic Factors
Smooth Muscle Myocytes
Smooth Muscle
Leukocytes
Receptors, Tumor Necrosis Factor, Type II
Cytokines
Messenger RNA
Glucocorticoid Receptors
Serine
Glucocorticoids
Phosphorylation
Fetal Proteins
Blocking Antibodies
Tumor Necrosis Factor Receptors
Neutralizing Antibodies
Fluticasone
Proteins
Lung

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

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Fetal human airway smooth muscle cell production of leukocyte chemoattractants is differentially regulated by fluticasone. / Pearson, Helen; Britt, Rodney Jr.; Pabelick, Christina M; Prakash, Y.s.; Amrani, Yassine; Pandya, Hitesh C.

In: Pediatric Research, Vol. 78, No. 6, 01.12.2015, p. 650-656.

Research output: Contribution to journalArticle

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AU - Amrani, Yassine

AU - Pandya, Hitesh C.

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N2 - Adult human airway smooth muscle (ASM) produce cytokines involved in recruitment and survival of leukocytes within airway walls. Cytokine generation by adult ASM is glucocorticoid-sensitive. Whether developing lung ASM produces cytokines in a glucocorticoid-sensitive fashion is unknown.Methods:Cultured fetal human ASM cells stimulated with TNF-α (0-20 ng/ml) were incubated with TNF-α receptor-blocking antibodies, fluticasone (1 and 100 nm), or vehicle. Supernatants and cells were assayed for the production of CCL5, CXCL10, and CXCL8 mRNA and protein and glucocorticoid receptor phosphorylation.Results:CCL5, CXCL10, and CXCL8 mRNA and protein production by fetal ASM cell was significantly and dose-dependently following TNF-α treatment. Cytokine mRNA and protein production were effectively blocked by TNF-α R1 and R2 receptor neutralizing antibodies but variably inhibited by fluticasone. TNF-α-induced TNF-R1 and R2 receptor mRNA expression was only partially attenuated by fluticasone. Glucocorticoid receptor phosphorylation at serine (Ser) 211 but not at Ser 226 was enhanced by fluticasone.Conclusion:Production of CCL5, CXCL10, and CXCL8 by fetal ASM appears to involve pathways that are both qualitatively and mechanistically distinct to those described for adult ASM. The findings imply developing ASM has potential to recruit leukocyte into airways and, therefore, of relevance to childhood airway diseases.

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