Fetal bovine serum and other sera used in tissue culture increase epithelial permeability

K. H. Mortell, Alan D Marmorstein, E. B. Cramer

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Fetal bovine serum (FBS) or heat-inactivated FBS (56°C for 30 min, HFBS) caused a dose-dependent decrease in the transepithelial electrical resistance of an epithelial monolayer (MDCK). A saturating concentration of HFBS (30%) caused an average fall of 25 ± 2% within 60 min. Upon removal of HFBS, the resistance returned to its starting value within 1 h. Flux studies with [3H]mannitol demonstrate that the fa]l in resistance is due to an increased permeability of the tight junctions. Thirty percent heat inactivated sera from goat, newborn calf, calf, bovine, and horse caused falls ranging from 26 to 47%. In contrast with the basolateral preference of human and bovine adult sera, fetal bovine and newborn calf sera elicit this response primarily by interacting with the apical surface of the epithelium. HFBS-treated monolayers show a significant increase in the condensation of F-actin at points where ≥3 cells meet. These results demonstrate that FBS and other sera used as nutritional supplements can increase the permeability of the tight junctions of cultured epithelial cells.

Original languageEnglish (US)
Pages (from-to)235-238
Number of pages4
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume29 A
Issue number3 I
StatePublished - 1993
Externally publishedYes

Fingerprint

Tissue culture
Monolayers
Permeability
Acoustic impedance
Mannitol
Serum
Actins
Condensation
Fluxes
Tight Junctions
Hot Temperature
Electric Impedance
Goats
Horses
Cultured Cells
Epithelium
Epithelial Cells

Keywords

  • Actin
  • Epithelial permeability factor
  • MDCK cells
  • Tight junctions

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Developmental Biology

Cite this

Fetal bovine serum and other sera used in tissue culture increase epithelial permeability. / Mortell, K. H.; Marmorstein, Alan D; Cramer, E. B.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 29 A, No. 3 I, 1993, p. 235-238.

Research output: Contribution to journalArticle

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