Ferritin in rat kidneys with specific lesions due to a single dose of lead

G. W. Richter, M. J. Velasquez, R. Shedd

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In the proximal tubular cells of rats or mice given a single, parenteral dose of lead, clusters of ferritin are frequently associated with characteristic cytoplasmic fibrillar bodies. To learn more about this relationship, the authors have investigated content and synthesis of ferritin protein and incorporation of iron into ferritin in rat kidneys 48 hours after a single parenteral dose of lead (10 μg/g). By immunoradiometric assays, they found that the kidneys of female rats, whether treated with lead or not, contained significantly more ferritin protein than did kidneys of males of the same age and provenance. Administration of lead diminished (or did not significantly alter) the incorporation of 14C-amino acids into newly synthesized ferritin protein. Contrary to expectation, administration of lead tended to depress incorporation of 59Fe into kidney ferritin in rats maintained on standard rations and distilled water. Electron microscopy confirmed the presence of clusters of ferritin in close association with dense fibrillar bodies in the cytoplasm of proximal tubular cells of rats given lead. Considered together, the findings indicate that clustering of ferritin next to the dense fibrillar cytoplasmic lesions is a selective effect of lead that requires neither augmented synthesis of ferritin protein nor increased incorporation of iron into preexisting ferritin.

Original languageEnglish (US)
Pages (from-to)483-495
Number of pages13
JournalAmerican Journal of Pathology
Volume94
Issue number3
StatePublished - Jan 1 1979

Fingerprint

Ferritins
Kidney
Proteins
Iron
Lead
Immunoradiometric Assay
Cluster Analysis
Electron Microscopy
Cytoplasm
Amino Acids
Water

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Ferritin in rat kidneys with specific lesions due to a single dose of lead. / Richter, G. W.; Velasquez, M. J.; Shedd, R.

In: American Journal of Pathology, Vol. 94, No. 3, 01.01.1979, p. 483-495.

Research output: Contribution to journalArticle

@article{f97578c04373491e840bb6192458ca31,
title = "Ferritin in rat kidneys with specific lesions due to a single dose of lead",
abstract = "In the proximal tubular cells of rats or mice given a single, parenteral dose of lead, clusters of ferritin are frequently associated with characteristic cytoplasmic fibrillar bodies. To learn more about this relationship, the authors have investigated content and synthesis of ferritin protein and incorporation of iron into ferritin in rat kidneys 48 hours after a single parenteral dose of lead (10 μg/g). By immunoradiometric assays, they found that the kidneys of female rats, whether treated with lead or not, contained significantly more ferritin protein than did kidneys of males of the same age and provenance. Administration of lead diminished (or did not significantly alter) the incorporation of 14C-amino acids into newly synthesized ferritin protein. Contrary to expectation, administration of lead tended to depress incorporation of 59Fe into kidney ferritin in rats maintained on standard rations and distilled water. Electron microscopy confirmed the presence of clusters of ferritin in close association with dense fibrillar bodies in the cytoplasm of proximal tubular cells of rats given lead. Considered together, the findings indicate that clustering of ferritin next to the dense fibrillar cytoplasmic lesions is a selective effect of lead that requires neither augmented synthesis of ferritin protein nor increased incorporation of iron into preexisting ferritin.",
author = "Richter, {G. W.} and Velasquez, {M. J.} and R. Shedd",
year = "1979",
month = "1",
day = "1",
language = "English (US)",
volume = "94",
pages = "483--495",
journal = "American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Ferritin in rat kidneys with specific lesions due to a single dose of lead

AU - Richter, G. W.

AU - Velasquez, M. J.

AU - Shedd, R.

PY - 1979/1/1

Y1 - 1979/1/1

N2 - In the proximal tubular cells of rats or mice given a single, parenteral dose of lead, clusters of ferritin are frequently associated with characteristic cytoplasmic fibrillar bodies. To learn more about this relationship, the authors have investigated content and synthesis of ferritin protein and incorporation of iron into ferritin in rat kidneys 48 hours after a single parenteral dose of lead (10 μg/g). By immunoradiometric assays, they found that the kidneys of female rats, whether treated with lead or not, contained significantly more ferritin protein than did kidneys of males of the same age and provenance. Administration of lead diminished (or did not significantly alter) the incorporation of 14C-amino acids into newly synthesized ferritin protein. Contrary to expectation, administration of lead tended to depress incorporation of 59Fe into kidney ferritin in rats maintained on standard rations and distilled water. Electron microscopy confirmed the presence of clusters of ferritin in close association with dense fibrillar bodies in the cytoplasm of proximal tubular cells of rats given lead. Considered together, the findings indicate that clustering of ferritin next to the dense fibrillar cytoplasmic lesions is a selective effect of lead that requires neither augmented synthesis of ferritin protein nor increased incorporation of iron into preexisting ferritin.

AB - In the proximal tubular cells of rats or mice given a single, parenteral dose of lead, clusters of ferritin are frequently associated with characteristic cytoplasmic fibrillar bodies. To learn more about this relationship, the authors have investigated content and synthesis of ferritin protein and incorporation of iron into ferritin in rat kidneys 48 hours after a single parenteral dose of lead (10 μg/g). By immunoradiometric assays, they found that the kidneys of female rats, whether treated with lead or not, contained significantly more ferritin protein than did kidneys of males of the same age and provenance. Administration of lead diminished (or did not significantly alter) the incorporation of 14C-amino acids into newly synthesized ferritin protein. Contrary to expectation, administration of lead tended to depress incorporation of 59Fe into kidney ferritin in rats maintained on standard rations and distilled water. Electron microscopy confirmed the presence of clusters of ferritin in close association with dense fibrillar bodies in the cytoplasm of proximal tubular cells of rats given lead. Considered together, the findings indicate that clustering of ferritin next to the dense fibrillar cytoplasmic lesions is a selective effect of lead that requires neither augmented synthesis of ferritin protein nor increased incorporation of iron into preexisting ferritin.

UR - http://www.scopus.com/inward/record.url?scp=0018388274&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018388274&partnerID=8YFLogxK

M3 - Article

C2 - 426036

AN - SCOPUS:0018388274

VL - 94

SP - 483

EP - 495

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 3

ER -