Feline immunodeficiency virus envelope glycoproteins antagonize tetherin through a distinctive mechanism that requires virion incorporation

James H. Morrison, Rebekah B. Guevara, Adriana C. Marcano, Dyana T. Saenz, Hind Fadel, Daniel K. Rogstad, Eric M. Poeschla

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

BST2/tetherin inhibits the release of enveloped viruses from cells. Primate lentiviruses have evolved specific antagonists (Vpu, Nef, and Env). Here we characterized tetherin proteins of species representing both branches of the order Carnivora. Comparison of tiger and cat (Feliformia) to dog and ferret (Caniformia) genes demonstrated that the tiger and cat share a start codon mutation that truncated most of the tetherin cytoplasmic tail early in the Feliformia lineage (19 of 27 amino acids, including the dual tyrosine motif). Alpha interferon (IFN-α) induced tetherin and blocked feline immunodeficiency virus (FIV) replication in lymphoid and nonlymphoid feline cells. Budding of bald FIV and HIV particles was blocked by carnivore tetherins. However, infectious FIV particles were resistant, and spreading FIV replication was uninhibited. Antagonism mapped to the envelope glycoprotein (Env), which rescued FIV from carnivore tetherin restriction when expressed in trans but, in contrast to known antagonists, did not rescue noncognate particles. Also unlike the primate lentiviral antagonists, but similar to the Ebola virus glycoprotein, FIV Env did not reduce intracellular or cell surface tetherin levels. Furthermore, FIV-enveloped FIV particles actually required tetherin for optimal release from cells. The results show that FIV Envs mediate a distinctive tetherin evasion. Well adapted to a phylogenetically ancient tetherin tail truncation in the Felidae, it requires functional virion incorporation of Env, and it shields the budding particle without downregulating plasma membrane tetherin. Moreover, FIV has evolved dependence on this protein: particles containing FIV Env need tetherin for optimal release from the cell, while Env- particles do not.

Original languageEnglish (US)
Pages (from-to)3255-3272
Number of pages18
JournalJournal of Virology
Volume88
Issue number6
DOIs
StatePublished - 2014

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Feline Immunodeficiency Virus
Feline immunodeficiency virus
virion
Virion
glycoproteins
Glycoproteins
Tigers
antagonists
Panthera tigris
Felidae
cats
Virus Replication
virus replication
carnivores
cells
Tail
Primates
Cats
tail
Primate Lentiviruses

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Feline immunodeficiency virus envelope glycoproteins antagonize tetherin through a distinctive mechanism that requires virion incorporation. / Morrison, James H.; Guevara, Rebekah B.; Marcano, Adriana C.; Saenz, Dyana T.; Fadel, Hind; Rogstad, Daniel K.; Poeschla, Eric M.

In: Journal of Virology, Vol. 88, No. 6, 2014, p. 3255-3272.

Research output: Contribution to journalArticle

Morrison, James H. ; Guevara, Rebekah B. ; Marcano, Adriana C. ; Saenz, Dyana T. ; Fadel, Hind ; Rogstad, Daniel K. ; Poeschla, Eric M. / Feline immunodeficiency virus envelope glycoproteins antagonize tetherin through a distinctive mechanism that requires virion incorporation. In: Journal of Virology. 2014 ; Vol. 88, No. 6. pp. 3255-3272.
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