Feline CD8+ cells induced with FIV infected, irradiated T cells produce multiple anti-FIV factors

Zhongxia Li, Anagha Phadke, Eric A. Weaver, Judith M. Ball, Ellen W. Collisson

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Feline immunodeficiency virus (FIV) infection in cats is the only non-primate, small animal model for HIV-AIDS. Replication of FIV has been shown to be optimally suppressed by soluble factors produced by inducer cell-stimulated feline CD8+ cells from FIV-infected cats. The nature of this dose-dependent suppression of FIV was examined. Antiviral factors, produced in serum-free medium, were shown to be either heat stable or heat labile. Suppressing activity was identified in a heparin-bound fraction and the non-bound fraction and in fractions separated by reverse-phase HPLC. The FIV suppression could not be correlated with IFN type I or II. Neither α nor β chemokines were likely candidates because molecular size exclusion centrifugation indicated that the major factors were larger than 50 kD. Identified qualitative differences in the properties of the soluble suppressive activity generated from feline lymphocytes indicated that multiple factors are responsible for the non-cytolytic CD8+ T cell suppression of FIV replication.

Original languageEnglish (US)
Pages (from-to)809-824
Number of pages16
JournalDevelopmental and Comparative Immunology
Volume29
Issue number9
DOIs
StatePublished - Jun 16 2005

Keywords

  • CD8 cell antiviral activity
  • FIV
  • Multiple factors

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

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