Fat depot origin affects fatty acid handling in cultured rat and human preadipocytes

Frank Caserta, Tamara Tchkonia, Vildan N. Civelek, Marc Prentki, Nicholas F. Brown, J. Denis McGarry, R. Armour Forse, Barbara E. Corkey, James A. Hamilton, James L Kirkland

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Regional differences in free fatty acid (FFA) handling contribute to diseases associated with particular fat distributions. As cultured rat preadipocytes became differentiated, FFA transfer into preadipocytes increased and was more rapid in single perirenal than in epididymal cells matched for lipid content. Uptake by human omental preadipocytes was greater than uptake by abdominal subcutaneous preadipocytes. Adipose-specific fatty acid binding protein (aP2) and keratinocyte lipid binding protein abundance was higher in differentiated rat perirenal than in epididymal preadipocytes. This interdepot difference in preadipocyte aP2 expression was reflected in fat tissue in older animals. Carnitine palmitoyltransferase 1 activity increased during differentiation and was higher in perirenal than in epididymal preadipocytes, particularly the muscle isoform. Long-chain acyl-CoA levels were higher in perirenal than in epididymal preadipocytes and isolated fat cells. These data are consistent with interdepot differences in fatty acid flux ensuing from differences in fatty acid binding proteins and enzymes of fat metabolism. Heterogeneity among depots results, in part, from distinct intrinsic characteristics of adipose cells. Different depots are effectively separate miniorgans.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume280
Issue number2 43-2
StatePublished - Feb 2001
Externally publishedYes

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Rats
Fatty Acid-Binding Proteins
Fatty Acids
Fats
Nonesterified Fatty Acids
Carnitine O-Palmitoyltransferase
Lipids
Acyl Coenzyme A
Keratinocytes
Adipocytes
Carrier Proteins
Protein Isoforms
Metabolism
Muscle
Muscles
Animals
Enzymes
Tissue
Fluxes

Keywords

  • Carnitine palmitoyltransferase 1
  • Fatty acid binding protein
  • Free fatty acid uptake
  • Regional fat distribution

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry
  • Physiology (medical)

Cite this

Fat depot origin affects fatty acid handling in cultured rat and human preadipocytes. / Caserta, Frank; Tchkonia, Tamara; Civelek, Vildan N.; Prentki, Marc; Brown, Nicholas F.; McGarry, J. Denis; Forse, R. Armour; Corkey, Barbara E.; Hamilton, James A.; Kirkland, James L.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 280, No. 2 43-2, 02.2001.

Research output: Contribution to journalArticle

Caserta, F, Tchkonia, T, Civelek, VN, Prentki, M, Brown, NF, McGarry, JD, Forse, RA, Corkey, BE, Hamilton, JA & Kirkland, JL 2001, 'Fat depot origin affects fatty acid handling in cultured rat and human preadipocytes', American Journal of Physiology - Endocrinology and Metabolism, vol. 280, no. 2 43-2.
Caserta F, Tchkonia T, Civelek VN, Prentki M, Brown NF, McGarry JD et al. Fat depot origin affects fatty acid handling in cultured rat and human preadipocytes. American Journal of Physiology - Endocrinology and Metabolism. 2001 Feb;280(2 43-2).
Caserta, Frank ; Tchkonia, Tamara ; Civelek, Vildan N. ; Prentki, Marc ; Brown, Nicholas F. ; McGarry, J. Denis ; Forse, R. Armour ; Corkey, Barbara E. ; Hamilton, James A. ; Kirkland, James L. / Fat depot origin affects fatty acid handling in cultured rat and human preadipocytes. In: American Journal of Physiology - Endocrinology and Metabolism. 2001 ; Vol. 280, No. 2 43-2.
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