Fast Cyclic Square-Wave Voltammetry to Enhance Neurotransmitter Selectivity and Sensitivity

Cheonho Park, Yoonbae Oh, Hojin Shin, Jaekyung Kim, Yumin Kang, Jeongeun Sim, Hyun U. Cho, Han Kyu Lee, Sung Jun Jung, Charles D. Blaha, Kevin E. Bennet, Michael L. Heien, Kendall H. Lee, In Young Kim, Dong Pyo Jang

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Although fast-scan cyclic voltammetry (FSCV) has been widely used for in vivo neurochemical detection, the sensitivity and selectivity of the technique can be further improved. In this study, we develop fast cyclic square-wave voltammetry (FCSWV) as a novel voltammetric technique that combines large-amplitude cyclic square-wave voltammetry (CSWV) with background subtraction. A large-amplitude, square-shaped potential was applied to induce cycling through multiple redox reactions within a square pulse to increase sensitivity and selectivity when combined with a two-dimensional voltammogram. As a result, FCSWV was significantly more sensitive than FSCV (n = 5 electrodes, two-way ANOVA, p = 0.0002). In addition, FCSWV could differentiate dopamine from other catecholamines (e.g., epinephrine and norepinephrine) and serotonin better than conventional FSCV. With the confirmation that FCSWV did not influence local neuronal activity, despite the large amplitude of the square waveform, it could monitor electrically induced phasic changes in dopamine release in rat striatum before and after injecting nomifensine, a dopamine reuptake inhibitor.

Original languageEnglish (US)
Pages (from-to)13348-13355
Number of pages8
JournalAnalytical Chemistry
Issue number22
StatePublished - Nov 20 2018

ASJC Scopus subject areas

  • Analytical Chemistry


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