Fas expression in lung metastasis from osteosarcoma patients

Nancy Gordon, Carola A.S. Arndt, Douglas S. Hawkins, Debra K. Doherty, Carrie Y. Inwards, Mark F. Munsell, John Stewart, Nadezhda V. Koshkina, Eugenie S. Kleinerman

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

The authors' animal studies have shown that the metastatic potential of osteosarcoma (OS) cells correlates inversely with Fas expression - that is, Fas-negative cells metastasize but Fas-positive cells do not. One reason for this in the context of OS lung metastases may be that Fas-positive cells are eliminated by engagement with the Fas ligand (FasL) constitutively expressed on the surface of pneumocytes, whereas Fas-negative tumor cells are not. The purpose of this study was to determine the status of Fas expression in OS lung metastases from patients. Specifically, archived paraffin-embedded specimens of lung metastases from 38 patients with OS were analyzed by immunohistochemistry. Lung nodules from 23 of the 38 patients (60%) were Fas negative, those from 12 patients (32%) were weakly positive, and that from only 1 patient (3%) was strongly positive. Findings in the samples from the remaining two patients (5%) could not be interpreted because of extensive necrosis. Most patients with the weakly positive tumors and the single patient with the strongly positive tumor received chemotherapy prior to lung resection. There was a significant correlation between Fas expression and the administration of preoperative salvage chemotherapy (P = 0.0013). These data indicate that loss of Fas may be one mechanism by which OS cells evade host resistance in the lung. Chemotherapy may induce regression by upregulating Fas.

Original languageEnglish (US)
Pages (from-to)611-615
Number of pages5
JournalJournal of Pediatric Hematology/Oncology
Volume27
Issue number11
DOIs
StatePublished - Nov 1 2005

Keywords

  • APO-1/Fas
  • Fas ligand
  • Osteosarcoma lung metastasis
  • Recent chemotherapy

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Hematology
  • Oncology

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  • Cite this

    Gordon, N., Arndt, C. A. S., Hawkins, D. S., Doherty, D. K., Inwards, C. Y., Munsell, M. F., Stewart, J., Koshkina, N. V., & Kleinerman, E. S. (2005). Fas expression in lung metastasis from osteosarcoma patients. Journal of Pediatric Hematology/Oncology, 27(11), 611-615. https://doi.org/10.1097/01.mph.0000188112.42576.df