FAM96A is a novel pro-apoptotic tumor suppressor in gastrointestinal stromal tumors

Bettina Schwamb, Robert Pick, Sara Beatriz Mateus Fernández, Kirsten Völp, Jan Heering, Volker Dötsch, Susanne Bösser, Jennifer Jung, Rasa Beinoraviciute-Kellner, Josephine Wesely, Inka Zörnig, Matthias Hammerschmidt, Matthias Nowak, Roland Penzel, Kurt Zatloukal, Stefan Joos, Ralf Joachim Rieker, Abbas Agaimy, Stephan Söder, Kmarie Reid-LombardoMichael L. Kendrick, Michael R. Bardsley, Yujiro Hayashi, David T. Asuzu, Sabriya A. Syed, Tamas Ordog, Martin Zörnig

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The ability to escape apoptosis is a hallmark of cancer-initiating cells and a key factor of resistance to oncolytic therapy. Here, we identify FAM96A as a ubiquitous, evolutionarily conserved apoptosome-activating protein and investigate its potential pro-apoptotic tumor suppressor function in gastrointestinal stromal tumors (GISTs). Interaction between FAM96A and apoptotic peptidase activating factor 1 (APAF1) was identified in yeast two-hybrid screen and further studied by deletion mutants, glutathione-S-transferase pull-down, co-immunoprecipitation and immunofluorescence. Effects of FAM96A overexpression and knock-down on apoptosis sensitivity were examined in cancer cells and zebrafish embryos. Expression of FAM96A in GISTs and histogenetically related cells including interstitial cells of Cajal (ICCs), "fibroblast-like cells" (FLCs) and ICC stem cells (ICC-SCs) was investigated by Northern blotting, reverse transcription - polymerase chain reaction, immunohistochemistry and Western immunoblotting. Tumorigenicity of GIST cells and transformed murine ICC-SCs stably transduced to re-express FAM96A was studied by xeno- and allografting into immunocompromised mice. FAM96A was found to bind APAF1 and to enhance the induction of mitochondrial apoptosis. FAM96A protein or mRNA was dramatically reduced or lost in 106 of 108 GIST samples representing three independent patient cohorts. Whereas ICCs, ICC-SCs and FLCs, the presumed normal counterparts of GIST, were found to robustly express FAM96A protein and mRNA, FAM96A expression was much reduced in tumorigenic ICC-SCs. Re-expression of FAM96A in GIST cells and transformed ICC-SCs increased apoptosis sensitivity and diminished tumorigenicity. Our data suggest FAM96A is a novel pro-apoptotic tumor suppressor that is lost during GIST tumorigenesis.

Original languageEnglish (US)
Pages (from-to)1318-1329
Number of pages12
JournalInternational Journal of Cancer
Volume137
Issue number6
DOIs
StatePublished - Sep 1 2015

Fingerprint

Gastrointestinal Stromal Tumors
Interstitial Cells of Cajal
Apoptotic Protease-Activating Factor 1
Neoplasms
Apoptosis
Stromal Cells
Apoptosomes
Fibroblasts
Messenger RNA
Heterologous Transplantation
Proteins
R Factors
Homologous Transplantation
Zebrafish
Glutathione Transferase
Immunoprecipitation
Northern Blotting
Reverse Transcription
Fluorescent Antibody Technique
Carcinogenesis

Keywords

  • apoptosis
  • FAM96A
  • GIST
  • ICC
  • tumor suppressor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Schwamb, B., Pick, R., Fernández, S. B. M., Völp, K., Heering, J., Dötsch, V., ... Zörnig, M. (2015). FAM96A is a novel pro-apoptotic tumor suppressor in gastrointestinal stromal tumors. International Journal of Cancer, 137(6), 1318-1329. https://doi.org/10.1002/ijc.29498

FAM96A is a novel pro-apoptotic tumor suppressor in gastrointestinal stromal tumors. / Schwamb, Bettina; Pick, Robert; Fernández, Sara Beatriz Mateus; Völp, Kirsten; Heering, Jan; Dötsch, Volker; Bösser, Susanne; Jung, Jennifer; Beinoraviciute-Kellner, Rasa; Wesely, Josephine; Zörnig, Inka; Hammerschmidt, Matthias; Nowak, Matthias; Penzel, Roland; Zatloukal, Kurt; Joos, Stefan; Rieker, Ralf Joachim; Agaimy, Abbas; Söder, Stephan; Reid-Lombardo, Kmarie; Kendrick, Michael L.; Bardsley, Michael R.; Hayashi, Yujiro; Asuzu, David T.; Syed, Sabriya A.; Ordog, Tamas; Zörnig, Martin.

In: International Journal of Cancer, Vol. 137, No. 6, 01.09.2015, p. 1318-1329.

Research output: Contribution to journalArticle

Schwamb, B, Pick, R, Fernández, SBM, Völp, K, Heering, J, Dötsch, V, Bösser, S, Jung, J, Beinoraviciute-Kellner, R, Wesely, J, Zörnig, I, Hammerschmidt, M, Nowak, M, Penzel, R, Zatloukal, K, Joos, S, Rieker, RJ, Agaimy, A, Söder, S, Reid-Lombardo, K, Kendrick, ML, Bardsley, MR, Hayashi, Y, Asuzu, DT, Syed, SA, Ordog, T & Zörnig, M 2015, 'FAM96A is a novel pro-apoptotic tumor suppressor in gastrointestinal stromal tumors', International Journal of Cancer, vol. 137, no. 6, pp. 1318-1329. https://doi.org/10.1002/ijc.29498
Schwamb B, Pick R, Fernández SBM, Völp K, Heering J, Dötsch V et al. FAM96A is a novel pro-apoptotic tumor suppressor in gastrointestinal stromal tumors. International Journal of Cancer. 2015 Sep 1;137(6):1318-1329. https://doi.org/10.1002/ijc.29498
Schwamb, Bettina ; Pick, Robert ; Fernández, Sara Beatriz Mateus ; Völp, Kirsten ; Heering, Jan ; Dötsch, Volker ; Bösser, Susanne ; Jung, Jennifer ; Beinoraviciute-Kellner, Rasa ; Wesely, Josephine ; Zörnig, Inka ; Hammerschmidt, Matthias ; Nowak, Matthias ; Penzel, Roland ; Zatloukal, Kurt ; Joos, Stefan ; Rieker, Ralf Joachim ; Agaimy, Abbas ; Söder, Stephan ; Reid-Lombardo, Kmarie ; Kendrick, Michael L. ; Bardsley, Michael R. ; Hayashi, Yujiro ; Asuzu, David T. ; Syed, Sabriya A. ; Ordog, Tamas ; Zörnig, Martin. / FAM96A is a novel pro-apoptotic tumor suppressor in gastrointestinal stromal tumors. In: International Journal of Cancer. 2015 ; Vol. 137, No. 6. pp. 1318-1329.
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