Failure to detect IL-3-binding sites on human mast cells

P. Valent, J. Besemer, C. Sillaber, J. H. Butterfield, R. Eher, O. Majdic, K. Kishi, W. Klepetko, F. Eckersberger, K. Lechner, P. Bettelheim

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Abstract

IL-3, a pleiotropic lymphokine, has been termed mast cell growth factor because it promotes growth and differentiation of murine mast cells. Murine mast cells, in turn, express cell surface receptors for IL-3. Human rIL-3 has been shown to induce proliferation and differentiation of human basophils and to activate basophils via high affinity binding sites. To investigate whether human mast cells express IL-3R, binding studies with 125I-radiolabeled human rIL-3 were performed on HMC-1, a novel human mast cell line, and on pure populations (i.e., 93 to 99% purity) of human tissue mast cells obtained with mAb and C from dispersed lung (n = 2). Unexpectedly, neither enriched human lung mast cells nor HMC-1 cells bound radiolabeled human rIL-3 specifically. Moreover, human rIL-3 failed to promote uptake of [3H]thymidine, synthesis of histamine, histamine releasability, or changes in expression of mast cell differentiation Ag (YB5B8, CD54/ICAM-1, CD9/p24, CD33/gp67) on either human lung mast cells or HMC-1 cells. It is hypothesized that the fundamental difference in the biologic response to IL-3 between human and murine mast cells is due to a loss during evolution of mast cell high affinity IL-3 binding sites.

Original languageEnglish (US)
Pages (from-to)3432-3437
Number of pages6
JournalJournal of Immunology
Volume145
Issue number10
StatePublished - Jan 1 1990

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Valent, P., Besemer, J., Sillaber, C., Butterfield, J. H., Eher, R., Majdic, O., Kishi, K., Klepetko, W., Eckersberger, F., Lechner, K., & Bettelheim, P. (1990). Failure to detect IL-3-binding sites on human mast cells. Journal of Immunology, 145(10), 3432-3437.