Failure of isolated rat tibial periosteal cells to 5α reduce testosterone to 5α‐dihydrotestosterone

Russell T. Turner, Batia Bleiberg, Douglas S. Colvard, Philip E. Keeting, Glenda Evans, Thomas C. Spelsberg

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Periosteal cells were isolated from tibiae of adult male rats after collagenase treatment. Northern blot analysis of total cytoplasmic RNA extracted from the isolated periosteal cells was positive for expression of genes encoding the osteoblast marker proteins osteocalcin (BGP) and pre‐pro‐α2(I) chain of type 1 procollagen. The isolated periosteal cells were incubated with 1 nM [3H]testosterone ([3H]T) for up to 240 minutes and the reaction products separated by high‐performance liquid chromatography. [3H]5α‐dihydrotestosterone ([3H]DHT) was not detected in extracts of periosteal cell incubations. In contrast, [3H]DHT was produced in a time‐dependent manner by cells from seminal vesicles. These results suggest that testosterone 5α‐reductase activity is not expressed by osteoblasts in rat tibial periosteum and that the anabolic effects of androgens in this tissue are not mediated by locally produced DHT.

Original languageEnglish (US)
Pages (from-to)775-779
Number of pages5
JournalJournal of Bone and Mineral Research
Volume5
Issue number7
DOIs
StatePublished - Jul 1990

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine

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