Failure of isolated rat tibial periosteal cells to 5α reduce testosterone to 5α-dihydrotestosterone

Russell T. Turner, Batia Bleiberg, Douglas S. Colvard, Philip E. Keeting, Glenda Evans, Thomas C. Spelsberg

Research output: Contribution to journalArticle

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Abstract

Periosteal cells were isolated from tibiae of adult male rats after collagenase treatment. Northern blot analysis of total cytoplasmic RNA extracted from the isolated periosteal cells was positive for expression of genes encoding the osteoblast marker proteins osteocalcin (BGP) and pre-pro-α2(I) chain of type 1 precollagen. The isolated periosteal cells were incubated with 1 nM [3H]testosterone ([3H]T) for up to 240 minutes and the reaction products separated by high-performance liquid chromatography. [3H]5α-dihydrotestosterone ([3H]DHT) was not detected in extracts of periosteal cell incubations. In contrast, [3H]DHT was produced in a time-dependent manner by cells from seminal vesicles. These results suggest that testosterone 5α-reductase activity is not expressed by osteoblasts in rat tibial periosteum and that the anabolic effects of androgens in this tissue are not mediated by locally produced DHT.

Original languageEnglish (US)
Pages (from-to)775-779
Number of pages5
JournalJournal of Bone and Mineral Research
Volume5
Issue number7
StatePublished - Jul 1990

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Dihydrotestosterone
Testosterone
Osteoblasts
Anabolic Agents
Periosteum
Seminal Vesicles
Osteocalcin
Collagenases
Cell Extracts
Tibia
Northern Blotting
Androgens
Oxidoreductases
High Pressure Liquid Chromatography
RNA
Gene Expression
Proteins

ASJC Scopus subject areas

  • Surgery

Cite this

Turner, R. T., Bleiberg, B., Colvard, D. S., Keeting, P. E., Evans, G., & Spelsberg, T. C. (1990). Failure of isolated rat tibial periosteal cells to 5α reduce testosterone to 5α-dihydrotestosterone. Journal of Bone and Mineral Research, 5(7), 775-779.

Failure of isolated rat tibial periosteal cells to 5α reduce testosterone to 5α-dihydrotestosterone. / Turner, Russell T.; Bleiberg, Batia; Colvard, Douglas S.; Keeting, Philip E.; Evans, Glenda; Spelsberg, Thomas C.

In: Journal of Bone and Mineral Research, Vol. 5, No. 7, 07.1990, p. 775-779.

Research output: Contribution to journalArticle

Turner, RT, Bleiberg, B, Colvard, DS, Keeting, PE, Evans, G & Spelsberg, TC 1990, 'Failure of isolated rat tibial periosteal cells to 5α reduce testosterone to 5α-dihydrotestosterone', Journal of Bone and Mineral Research, vol. 5, no. 7, pp. 775-779.
Turner RT, Bleiberg B, Colvard DS, Keeting PE, Evans G, Spelsberg TC. Failure of isolated rat tibial periosteal cells to 5α reduce testosterone to 5α-dihydrotestosterone. Journal of Bone and Mineral Research. 1990 Jul;5(7):775-779.
Turner, Russell T. ; Bleiberg, Batia ; Colvard, Douglas S. ; Keeting, Philip E. ; Evans, Glenda ; Spelsberg, Thomas C. / Failure of isolated rat tibial periosteal cells to 5α reduce testosterone to 5α-dihydrotestosterone. In: Journal of Bone and Mineral Research. 1990 ; Vol. 5, No. 7. pp. 775-779.
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