Factors in standardizing automated cholinesterase assays

Barry W. Wilson, Stephanie Padilla, John D. Henderson, William Stephen Brimijoin, Proveen D. Dass, Glenn Elliot, Bruce Jaeger, David Lanz, Renee Pearson, Rachel Spies

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

A scientific panel assembled by the U.S. Environmental Protection Agency (EPA) determined that variability in cholinesterase (ChE) activities in the agency's pesticide/animal study database likely was due to a lack of accepted guidelines for ChE methodology. A series of trials was held in which participating laboratories measured ChE activity in blood and brain samples from untreated and pesticide-treated rats using a colorimetric assay method. The degree of inhibition of ChE activity in plasma and brain samples compared to controls was consistent among most of the laboratories. The ChE activity in erythrocyte samples differed more between laboratories due to a high blank, low erythrocyte AChE activity and hemoglobin absorption at the wavelength of the assay. Strategies are suggested for minimizing the variability of ChE activity in hemoglobin-rich samples.

Original languageEnglish (US)
Pages (from-to)187-196
Number of pages10
JournalJournal of Toxicology and Environmental Health
Volume48
Issue number2
DOIs
StatePublished - Jan 1 1996

Fingerprint

Cholinesterases
Assays
Hemoglobin
Pesticides
Brain
Environmental Protection Agency
Rats
Hemoglobins
Animals
Blood
Erythrocytes
Plasmas
United States Environmental Protection Agency
Wavelength
Databases
Guidelines

ASJC Scopus subject areas

  • Toxicology
  • Pollution

Cite this

Wilson, B. W., Padilla, S., Henderson, J. D., Brimijoin, W. S., Dass, P. D., Elliot, G., ... Spies, R. (1996). Factors in standardizing automated cholinesterase assays. Journal of Toxicology and Environmental Health, 48(2), 187-196. https://doi.org/10.1080/009841096161429

Factors in standardizing automated cholinesterase assays. / Wilson, Barry W.; Padilla, Stephanie; Henderson, John D.; Brimijoin, William Stephen; Dass, Proveen D.; Elliot, Glenn; Jaeger, Bruce; Lanz, David; Pearson, Renee; Spies, Rachel.

In: Journal of Toxicology and Environmental Health, Vol. 48, No. 2, 01.01.1996, p. 187-196.

Research output: Contribution to journalArticle

Wilson, BW, Padilla, S, Henderson, JD, Brimijoin, WS, Dass, PD, Elliot, G, Jaeger, B, Lanz, D, Pearson, R & Spies, R 1996, 'Factors in standardizing automated cholinesterase assays', Journal of Toxicology and Environmental Health, vol. 48, no. 2, pp. 187-196. https://doi.org/10.1080/009841096161429
Wilson, Barry W. ; Padilla, Stephanie ; Henderson, John D. ; Brimijoin, William Stephen ; Dass, Proveen D. ; Elliot, Glenn ; Jaeger, Bruce ; Lanz, David ; Pearson, Renee ; Spies, Rachel. / Factors in standardizing automated cholinesterase assays. In: Journal of Toxicology and Environmental Health. 1996 ; Vol. 48, No. 2. pp. 187-196.
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