Face-selective adhesion of calcium oxalate dihydrate crystals to renal epithelial cells

John C Lieske, F. G. Toback, S. Deganello

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The interaction between the most common urinary crystal, calcium oxalate dihydrate (COD) and the surface of monkey renal epithelial cells of the BSC- 1 line was investigated. The {100} face of exogenous COD crystals bound selectively and rapidly to the kidney cell surface. Cellular processes extended preferentially over the {100} face initially, and then progressively covered the crystal so that by 24 hours some crystals were observed beneath the plasma membrane. When nucleated from solution onto the surface of the cell monolayer, COD crystals oriented preferentially so that their {100} face were in direct contact with the cell surface. In contrast, when siliconized glass was used as a substrate, nucleated COD crystals oriented randomly. Therefore, structures on the apical surface of renal tubular cells that mediate a stereospecific interaction with the molecular array presented by the {100} face of a COD crystal may be important determinants of crystal adhesion that could contribute to crystal retention and formation of kidney stones.

Original languageEnglish (US)
Pages (from-to)195-200
Number of pages6
JournalCalcified Tissue International
Volume58
Issue number3
DOIs
StatePublished - 1996
Externally publishedYes

Fingerprint

Calcium Oxalate
Epithelial Cells
Kidney
Kidney Calculi
Haplorhini
Glass
Cell Membrane

Keywords

  • Crystal nucleation
  • Kidney tubule
  • Nephrolithiasis
  • Plasma membrane
  • Stereospecificity

ASJC Scopus subject areas

  • Endocrinology

Cite this

Face-selective adhesion of calcium oxalate dihydrate crystals to renal epithelial cells. / Lieske, John C; Toback, F. G.; Deganello, S.

In: Calcified Tissue International, Vol. 58, No. 3, 1996, p. 195-200.

Research output: Contribution to journalArticle

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