Abstract
The importance of nanotechnology in human health care is unquestionable. There is a growing interest to apply nanotechnology in cancer therapy, as an example. In this report, we have fabricated gold nanoparticle (AuNP), the delivery vehicle of interest, with an anti-cancer drug (Velcade) and a targeting agent (folic acid) for potential therapeutic application in multiple myeloma. Physico-chemical characterization of the nanoconjugates were carried out by UV-Visble spectroscopy exploiting the surface plasmon resonance band of AuNP, transmission electron microscopy (TEM) and studying release of radio-labeled tritiated folic acid (3FA) in a scintillation counter. The evidence of the attachment of velcade to AuNP was confirmed UV-Vis spectroscopy and studying the release of 3FA from gold-velcade-FA conjugates (Au-Vel-FA). Furthermore, the stability of Au-Vel-FA tested in PBS (phosphate buffer saline) by monitoring the release of 3FA suggested significant stability of the nanoconjugate. Functional activity of the nanoconjugate was tested by studying the apoptosis of different multiple myeloma cell lines by annexin/PI method. Apoptosis data suggested that Velcade retained its functional activity in Au-Vel-FA. In future, this strategy may be potentially utilized as an alternative approach for the treatment of multiple myeloma.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 499-507 |
| Number of pages | 9 |
| Journal | Journal of Biomedical Nanotechnology |
| Volume | 4 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 2008 |
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Keywords
- Drug delivery
- Gold nanoparticle
- Multiple myeloma
- Velcade
ASJC Scopus subject areas
- Materials Science(all)
- Bioengineering
- Biomedical Engineering
- Medicine (miscellaneous)
- Pharmaceutical Science
Cite this
Fabrication of gold nanoparticle for potential application in multiple myeloma. / Patra, Chitta Ranjan; Verma, Rajanshu; Kumar, Shaji K; Greipp, Philip R.; Mukhopadhyay, Debabrata; Mukherjee, Priyabrata.
In: Journal of Biomedical Nanotechnology, Vol. 4, No. 4, 12.2008, p. 499-507.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fabrication of gold nanoparticle for potential application in multiple myeloma
AU - Patra, Chitta Ranjan
AU - Verma, Rajanshu
AU - Kumar, Shaji K
AU - Greipp, Philip R.
AU - Mukhopadhyay, Debabrata
AU - Mukherjee, Priyabrata
PY - 2008/12
Y1 - 2008/12
N2 - The importance of nanotechnology in human health care is unquestionable. There is a growing interest to apply nanotechnology in cancer therapy, as an example. In this report, we have fabricated gold nanoparticle (AuNP), the delivery vehicle of interest, with an anti-cancer drug (Velcade) and a targeting agent (folic acid) for potential therapeutic application in multiple myeloma. Physico-chemical characterization of the nanoconjugates were carried out by UV-Visble spectroscopy exploiting the surface plasmon resonance band of AuNP, transmission electron microscopy (TEM) and studying release of radio-labeled tritiated folic acid (3FA) in a scintillation counter. The evidence of the attachment of velcade to AuNP was confirmed UV-Vis spectroscopy and studying the release of 3FA from gold-velcade-FA conjugates (Au-Vel-FA). Furthermore, the stability of Au-Vel-FA tested in PBS (phosphate buffer saline) by monitoring the release of 3FA suggested significant stability of the nanoconjugate. Functional activity of the nanoconjugate was tested by studying the apoptosis of different multiple myeloma cell lines by annexin/PI method. Apoptosis data suggested that Velcade retained its functional activity in Au-Vel-FA. In future, this strategy may be potentially utilized as an alternative approach for the treatment of multiple myeloma.
AB - The importance of nanotechnology in human health care is unquestionable. There is a growing interest to apply nanotechnology in cancer therapy, as an example. In this report, we have fabricated gold nanoparticle (AuNP), the delivery vehicle of interest, with an anti-cancer drug (Velcade) and a targeting agent (folic acid) for potential therapeutic application in multiple myeloma. Physico-chemical characterization of the nanoconjugates were carried out by UV-Visble spectroscopy exploiting the surface plasmon resonance band of AuNP, transmission electron microscopy (TEM) and studying release of radio-labeled tritiated folic acid (3FA) in a scintillation counter. The evidence of the attachment of velcade to AuNP was confirmed UV-Vis spectroscopy and studying the release of 3FA from gold-velcade-FA conjugates (Au-Vel-FA). Furthermore, the stability of Au-Vel-FA tested in PBS (phosphate buffer saline) by monitoring the release of 3FA suggested significant stability of the nanoconjugate. Functional activity of the nanoconjugate was tested by studying the apoptosis of different multiple myeloma cell lines by annexin/PI method. Apoptosis data suggested that Velcade retained its functional activity in Au-Vel-FA. In future, this strategy may be potentially utilized as an alternative approach for the treatment of multiple myeloma.
KW - Drug delivery
KW - Gold nanoparticle
KW - Multiple myeloma
KW - Velcade
UR - http://www.scopus.com/inward/record.url?scp=58149333519&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149333519&partnerID=8YFLogxK
U2 - 10.1166/jbn.2008.015
DO - 10.1166/jbn.2008.015
M3 - Article
AN - SCOPUS:58149333519
VL - 4
SP - 499
EP - 507
JO - Journal of Biomedical Nanotechnology
JF - Journal of Biomedical Nanotechnology
SN - 1550-7033
IS - 4
ER -