TY - JOUR
T1 - Extrinsic denervation causes a transient proabsorptive adrenergic hypersensitivity in the canine proximal colon
AU - Kendrick, Michael L.
AU - Meile, Tobias
AU - Zyromski, Nicholas J.
AU - Tanaka, Toshiyuki
AU - Libsch, Karen D.
AU - Sarr, Michael G.
PY - 2002
Y1 - 2002
N2 - Our aim was to determine if extrinsic denervation alters the absorptive response of the colon to proabsorptive and prosecretory stimuli. Ten dogs underwent enteric isolation of a 50-cm proximal colonic segment; five were also randomized to undergo extrinsic denervation (DEN). At 2 and 13 wk postoperatively, net absorptive fluxes (mean ± SEM) of water and electrolytes were determined during basal conditions and during proabsorptive low-dose (0.3 μg/kg/min) or high-dose (3 μg/kg/min) norepinephrine or prosecretory VIP (500 pg/kg/min). The net absorptive flux of water under basal conditions was decreased in DEN versus neurally intact controls at two weeks (4.0 ± 0.6 vs 6.6 ± 0.7 μl/min/cm, P = 0.03) but did not differ at 13 weeks(5.0 ± 1.0 vs 5.7 ± 0.9, P > 0.05). Low- and high-dose norepinephrine increased water absorption in both groups at two weeks; the change in flux for high-dose norepinephrine was greater in DEN versus controls (4.1 ± 1 vs 2.1 ± 0.6 μl/min/cm, P = 0.04). Net absorptive fluxes of Na+ and Cl- followed these trends. VIP did not alter absorption of water or electrolytes. Extrinsic denervation of the proximal colon causes a decrease in net colonic absorption and a transient, proabsorptive adrenergic hypersensitivity in colonic absorption of water and electrolytes. VIP does not have a net secretory effect in the proximal canine colon.
AB - Our aim was to determine if extrinsic denervation alters the absorptive response of the colon to proabsorptive and prosecretory stimuli. Ten dogs underwent enteric isolation of a 50-cm proximal colonic segment; five were also randomized to undergo extrinsic denervation (DEN). At 2 and 13 wk postoperatively, net absorptive fluxes (mean ± SEM) of water and electrolytes were determined during basal conditions and during proabsorptive low-dose (0.3 μg/kg/min) or high-dose (3 μg/kg/min) norepinephrine or prosecretory VIP (500 pg/kg/min). The net absorptive flux of water under basal conditions was decreased in DEN versus neurally intact controls at two weeks (4.0 ± 0.6 vs 6.6 ± 0.7 μl/min/cm, P = 0.03) but did not differ at 13 weeks(5.0 ± 1.0 vs 5.7 ± 0.9, P > 0.05). Low- and high-dose norepinephrine increased water absorption in both groups at two weeks; the change in flux for high-dose norepinephrine was greater in DEN versus controls (4.1 ± 1 vs 2.1 ± 0.6 μl/min/cm, P = 0.04). Net absorptive fluxes of Na+ and Cl- followed these trends. VIP did not alter absorption of water or electrolytes. Extrinsic denervation of the proximal colon causes a decrease in net colonic absorption and a transient, proabsorptive adrenergic hypersensitivity in colonic absorption of water and electrolytes. VIP does not have a net secretory effect in the proximal canine colon.
KW - Adrenergic hypersensitivity
KW - Colonic absorption
KW - Electrolyte absorption
KW - Extrinsic denervation
KW - Extrinsic innervation
KW - Intestinal transplantation
KW - Norepinephrine
KW - Vasoactive intestinal peptide
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U2 - 10.1023/A:1016436310180
DO - 10.1023/A:1016436310180
M3 - Article
C2 - 12184526
AN - SCOPUS:0036335811
SN - 0163-2116
VL - 47
SP - 1752
EP - 1757
JO - Digestive diseases and sciences
JF - Digestive diseases and sciences
IS - 8
ER -