Extraction of glyceric and glycolic acids from urine with tetrahydrofuran

Utility in detection of primary hyperoxaluria

D. J. Dietzen, T. R. Wilhite, D. N. Kenagy, D. S. Milliner, C. H. Smith, M. Landt

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Primary hyperoxaluria (PH) is an autosomal recessive metabolic abnormality characterized by excessive oxalate excretion leading to nephrocalcinosis and progressive renal dysfunction. Type I primary hyperoxaluria (PH I) results from a deficiency of alanine:glyoxylate aminotransferase, whereas type II disease has been traced to a deficiency of D-glycerate dehydrogenase. The two syndromes are often distinguished on the basis of organic acids that are coexcreted with oxalate: glycolate and L- glycerate in type I and type II disease, respectively. Routine organic acid analysis with diethyl ether extraction followed by gas chromatographic analysis failed to detect normal and increased concentrations of these diagnostic metabolites. Subsequent extraction of urine with tetrahydrofuran (THF), however, extracted 75% of added glycerate, 42% of added glycolate, and 75% of added ethylphosphonic acid (internal calibrator). THF extraction was analytically sensitive enough to allow determination of normal excretion of glycolate (14-72 μg/mg creatinine) and glycerate (0-5 years, 12177 μg/mg creatinine and >5 years, 19-115 μg/mg creatinine). Four of five patients with PHI and both patients with type II disease were correctly identified. Thus, THF extraction is a convenient adjunct to routine organic acid analysis and facilitates the detection of PH.

Original languageEnglish (US)
Pages (from-to)1315-1320
Number of pages6
JournalClinical Chemistry
Volume43
Issue number8
StatePublished - 1997
Externally publishedYes

Fingerprint

glycolic acid
Glyceric Acids
Primary Hyperoxaluria
Organic acids
Urine
Creatinine
Acids
Oxalates
Glycerate dehydrogenase
Nephrocalcinosis
Metabolites
Gas chromatography
Gas Chromatography
Ether
Kidney
tetrahydrofuran

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Dietzen, D. J., Wilhite, T. R., Kenagy, D. N., Milliner, D. S., Smith, C. H., & Landt, M. (1997). Extraction of glyceric and glycolic acids from urine with tetrahydrofuran: Utility in detection of primary hyperoxaluria. Clinical Chemistry, 43(8), 1315-1320.

Extraction of glyceric and glycolic acids from urine with tetrahydrofuran : Utility in detection of primary hyperoxaluria. / Dietzen, D. J.; Wilhite, T. R.; Kenagy, D. N.; Milliner, D. S.; Smith, C. H.; Landt, M.

In: Clinical Chemistry, Vol. 43, No. 8, 1997, p. 1315-1320.

Research output: Contribution to journalArticle

Dietzen, DJ, Wilhite, TR, Kenagy, DN, Milliner, DS, Smith, CH & Landt, M 1997, 'Extraction of glyceric and glycolic acids from urine with tetrahydrofuran: Utility in detection of primary hyperoxaluria', Clinical Chemistry, vol. 43, no. 8, pp. 1315-1320.
Dietzen DJ, Wilhite TR, Kenagy DN, Milliner DS, Smith CH, Landt M. Extraction of glyceric and glycolic acids from urine with tetrahydrofuran: Utility in detection of primary hyperoxaluria. Clinical Chemistry. 1997;43(8):1315-1320.
Dietzen, D. J. ; Wilhite, T. R. ; Kenagy, D. N. ; Milliner, D. S. ; Smith, C. H. ; Landt, M. / Extraction of glyceric and glycolic acids from urine with tetrahydrofuran : Utility in detection of primary hyperoxaluria. In: Clinical Chemistry. 1997 ; Vol. 43, No. 8. pp. 1315-1320.
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