Extracellular virulence factors in Bacillus cereus endophthalmitis: Methods and implication of involvement of hemolysin BL

D. J. Beecher, Jose S Pulido, N. P. Barney, A. C. Lee Wong

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81 Citations (Scopus)

Abstract

Bacillus cereus is a common cause of highly fulminant posttraumatic and metastatic endophthalmitis. Exotoxins or enzymes likely contribute to the severity of the infection, but specific virulence factors have not been identified. We developed two methods for the identification of B. cereus ocular virulence factors. In an in vitro assay that allows screening of multiple samples, retinal toxicity was estimated by measuring the release of lactate dehydrogenase from retinal buttons treated with B. cereus toxins. The results from this assay were confirmed with a sterile endophthalmitis model in which the histopathologic effect of intravitreally injected toxins was assessed. We tested pure hemolysin BL (HBL), a tripartite dermonecrotic vascular permeability factor of B. cereus, and crude exotoxin (GET) preparations, consisting of concentrated, cell-free B. cereus culture supernatant. In the in vitro assay, both GET and HBL caused rapid release of lactate dehydrogenase and retinal disintegration. In vivo, the toxins caused endophthalmitis clinically characteristic of B. cereus within 4 h. Histological changes included rapid retinal necrosis and detachment, choroidal edema, detachment and disruption of the retinal pigment epithelium, and rapid infiltration of polymorphonuclear leukocytes. Neutralization of HBL in CET preparations inhibited toxicity in vitro by 54%, and pure HBL was less toxic than CET with equal HBL contents in both methods. The results suggest that B. cereus ocular virulence is multifactorial and that HBL contributes to virulence.

Original languageEnglish (US)
Pages (from-to)632-639
Number of pages8
JournalInfection and Immunity
Volume63
Issue number2
StatePublished - 1995
Externally publishedYes

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Hemolysin Proteins
Endophthalmitis
Bacillus cereus
Virulence Factors
Exotoxins
L-Lactate Dehydrogenase
Virulence
Retinal Pigment Epithelium
Poisons
Retinal Detachment
Vascular Endothelial Growth Factor A
Edema
Neutrophils
Necrosis
Enzymes
Infection
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

Extracellular virulence factors in Bacillus cereus endophthalmitis : Methods and implication of involvement of hemolysin BL. / Beecher, D. J.; Pulido, Jose S; Barney, N. P.; Lee Wong, A. C.

In: Infection and Immunity, Vol. 63, No. 2, 1995, p. 632-639.

Research output: Contribution to journalArticle

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