Extracellular vesicles from osteosarcoma cell lines contain miRNAs associated with cell adhesion and apoptosis

Sofía Jerez, Héctor Araya, Daniel Hevia, Carlos E. Irarrázaval, Roman Thaler, Andre J van Wijnen, Mario Galindo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Osteosarcoma is the most common primary bone tumor during childhood and adolescence. Several reports have presented data on serum biomarkers for osteosarcoma, but few reports have analyzed circulating microRNAs (miRNAs). In this study, we used next generation miRNA sequencing to examine miRNAs isolated from microvesicle-depleted extracellular vesicles (EVs) derived from six different human osteosarcoma or osteoblastic cell lines with different degrees of metastatic potential (i.e., SAOS2, MG63, HOS, 143B, U2OS and hFOB1.19). EVs from each cell line contain on average ~300 miRNAs, and ~70 of these miRNAs are present at very high levels (i.e., >1000 reads per million). The most prominent miRNAs are miR-21-5p, miR-143-3p, miR-148a-3p and 181a-5p, which are enriched between 3 and 100 fold and relatively abundant in EVs derived from metastatic SAOS2 cells compared to non-metastatic MG63 cells. Gene ontology analysis of predicted targets reveals that miRNAs present in EVs may regulate the metastatic potential of osteosarcoma cell lines by potentially inhibiting a network of genes (e.g., MAPK1, NRAS, FRS2, PRCKE, BCL2 and QKI) involved in apoptosis and/or cell adhesion. Our data indicate that osteosarcoma cell lines may selectively package miRNAs as molecular cargo of EVs that could function as paracrine agents to modulate the tumor micro-environment.

Original languageEnglish (US)
Pages (from-to)246-257
Number of pages12
JournalGene
Volume710
DOIs
StatePublished - Aug 20 2019

Fingerprint

Osteosarcoma
MicroRNAs
Cell Adhesion
Apoptosis
Cell Line
Gene Ontology
Extracellular Vesicles
Gene Regulatory Networks
Neoplasms
Biomarkers
Bone and Bones
Serum

Keywords

  • Cancer
  • Cell aggressiveness
  • Exosomes
  • Metastasis
  • MicroRNA
  • Osteosarcoma

ASJC Scopus subject areas

  • Genetics

Cite this

Jerez, S., Araya, H., Hevia, D., Irarrázaval, C. E., Thaler, R., van Wijnen, A. J., & Galindo, M. (2019). Extracellular vesicles from osteosarcoma cell lines contain miRNAs associated with cell adhesion and apoptosis. Gene, 710, 246-257. https://doi.org/10.1016/j.gene.2019.06.005

Extracellular vesicles from osteosarcoma cell lines contain miRNAs associated with cell adhesion and apoptosis. / Jerez, Sofía; Araya, Héctor; Hevia, Daniel; Irarrázaval, Carlos E.; Thaler, Roman; van Wijnen, Andre J; Galindo, Mario.

In: Gene, Vol. 710, 20.08.2019, p. 246-257.

Research output: Contribution to journalArticle

Jerez, S, Araya, H, Hevia, D, Irarrázaval, CE, Thaler, R, van Wijnen, AJ & Galindo, M 2019, 'Extracellular vesicles from osteosarcoma cell lines contain miRNAs associated with cell adhesion and apoptosis', Gene, vol. 710, pp. 246-257. https://doi.org/10.1016/j.gene.2019.06.005
Jerez S, Araya H, Hevia D, Irarrázaval CE, Thaler R, van Wijnen AJ et al. Extracellular vesicles from osteosarcoma cell lines contain miRNAs associated with cell adhesion and apoptosis. Gene. 2019 Aug 20;710:246-257. https://doi.org/10.1016/j.gene.2019.06.005
Jerez, Sofía ; Araya, Héctor ; Hevia, Daniel ; Irarrázaval, Carlos E. ; Thaler, Roman ; van Wijnen, Andre J ; Galindo, Mario. / Extracellular vesicles from osteosarcoma cell lines contain miRNAs associated with cell adhesion and apoptosis. In: Gene. 2019 ; Vol. 710. pp. 246-257.
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