Extracellular ATP enhances radiation-induced brain injury through microglial activation and paracrine signaling via P2X7 receptor

Pengfei Xu, Yongteng Xu, Bin Hu, Jue Wang, Rui Pan, Madhuvika Murugan, LongJun Wu, Yamei Tang

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Activation of purinergic receptors by extracellular ATP (eATP) released from injured cells has been implicated in the pathogenesis of many neuronal disorders. The P2X7 receptor (P2X7R), an ion-selective purinergic receptor, is associated with microglial activation and paracrine signaling. However, whether ATP and P2X7R are involved in radiation-induced brain injury (RBI) remains to be determined. Here, we found that the eATP level was elevated in the cerebrospinal fluid (CSF) of RBI patients and was associated with the clinical severity of the disorder. In our experimental model, radiation treatment increased the level of eATP in the supernatant of primary cultures of neurons and glial cells and in the CSF of irradiated mice. In addition, ATP administration activated microglia, induced the release of the inflammatory mediators such as cyclooxygenase-2, tumor necrosis factor α and interleukin 6, and promoted neuronal apoptosis. Furthermore, blockade of ATP-P2X7R interaction using P2X7 antagonist Brilliant Blue G or P2X7 knockdown suppressed radiation-induced microglial activation and proliferation in the hippocampus, and restored the spatial memory of irradiated mice. Finally, we found that the PI3K/AKT and nuclear factor κB mediated pathways were downstream of ATP-P2X7R signaling in RBI. Taken together, our results unveiled the critical role of ATP-P2X7R in brain damage in RBI, suggesting that inhibition of ATP-P2X7R axis might be a potential strategy for the treatment of patients with RBI.

Original languageEnglish (US)
Pages (from-to)87-100
Number of pages14
JournalBrain, Behavior, and Immunity
Volume50
DOIs
StatePublished - Nov 1 2015
Externally publishedYes

Fingerprint

Paracrine Communication
Purinergic P2X7 Receptors
Purinergic P2 Receptors
Brain Injuries
Adenosine Triphosphate
Radiation
Purinergic Receptors
Cerebrospinal Fluid
Microglia
Cyclooxygenase 2
Phosphatidylinositol 3-Kinases
Neuroglia
Interleukin-6
Hippocampus
Theoretical Models
Tumor Necrosis Factor-alpha
Ions
Apoptosis
Neurons
Brain

Keywords

  • BBG
  • COX-2
  • Extracellular ATP
  • IL-6
  • Inflammation
  • Microglia
  • P2X7R
  • Radiation-induced brain injury
  • TNF-α

ASJC Scopus subject areas

  • Immunology
  • Endocrine and Autonomic Systems
  • Behavioral Neuroscience

Cite this

Extracellular ATP enhances radiation-induced brain injury through microglial activation and paracrine signaling via P2X7 receptor. / Xu, Pengfei; Xu, Yongteng; Hu, Bin; Wang, Jue; Pan, Rui; Murugan, Madhuvika; Wu, LongJun; Tang, Yamei.

In: Brain, Behavior, and Immunity, Vol. 50, 01.11.2015, p. 87-100.

Research output: Contribution to journalArticle

Xu, Pengfei ; Xu, Yongteng ; Hu, Bin ; Wang, Jue ; Pan, Rui ; Murugan, Madhuvika ; Wu, LongJun ; Tang, Yamei. / Extracellular ATP enhances radiation-induced brain injury through microglial activation and paracrine signaling via P2X7 receptor. In: Brain, Behavior, and Immunity. 2015 ; Vol. 50. pp. 87-100.
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