Extracellular acidification parallels insulin secretion in INS-1 and HIT-T15 β-cell lines

Douglas B. Luckie, John H. Wilterding, Joseph J. Maleszewski, Errett C. Hobbs, L. Karl Olson

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

As an alternative to manual assays that track insulin secretion, we tested a silicon-based biosensor that allows automated monitoring of extracellular acidification. Glucose stimulation of INS-1 and HIT-T15 cells resulted in a rapid increase in extracellular acidification in a biphasic and concentration-dependent fashion much like insulin secretion (EC50 INS-1 = 5 mM and HIT-T15 = 1 mM). This response was attenuated by verapamil (10 μM) and stimulated by administration of glybenclamide (100 nM) or KCl-induced (40 mM) depolarization. These experiments suggest that automated monitoring of extracellular pH may be a useful assay and support the relevance of linking metabolic activity to insulin secretion.

Original languageEnglish (US)
Pages (from-to)1168-1173
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume293
Issue number4
DOIs
StatePublished - Jul 4 2002

Keywords

  • Biphasic
  • Cytosensor
  • Diabetes
  • Microphysiometry
  • pH

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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