Extracellular acidification parallels insulin secretion in INS-1 and HIT-T15 β-cell lines

Douglas B. Luckie, John H. Wilterding, Joseph Maleszewski, Errett C. Hobbs, L. Karl Olson

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

As an alternative to manual assays that track insulin secretion, we tested a silicon-based biosensor that allows automated monitoring of extracellular acidification. Glucose stimulation of INS-1 and HIT-T15 cells resulted in a rapid increase in extracellular acidification in a biphasic and concentration-dependent fashion much like insulin secretion (EC50 INS-1 = 5 mM and HIT-T15 = 1 mM). This response was attenuated by verapamil (10 μM) and stimulated by administration of glybenclamide (100 nM) or KCl-induced (40 mM) depolarization. These experiments suggest that automated monitoring of extracellular pH may be a useful assay and support the relevance of linking metabolic activity to insulin secretion.

Original languageEnglish (US)
Pages (from-to)1168-1173
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume293
Issue number4
DOIs
StatePublished - Jul 4 2002
Externally publishedYes

Fingerprint

Acidification
Cells
Insulin
Cell Line
Assays
Monitoring
Glyburide
Depolarization
Biosensing Techniques
Silicon
Verapamil
Biosensors
Glucose
Experiments

Keywords

  • Biphasic
  • Cytosensor
  • Diabetes
  • Microphysiometry
  • pH

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Extracellular acidification parallels insulin secretion in INS-1 and HIT-T15 β-cell lines. / Luckie, Douglas B.; Wilterding, John H.; Maleszewski, Joseph; Hobbs, Errett C.; Olson, L. Karl.

In: Biochemical and Biophysical Research Communications, Vol. 293, No. 4, 04.07.2002, p. 1168-1173.

Research output: Contribution to journalArticle

Luckie, Douglas B. ; Wilterding, John H. ; Maleszewski, Joseph ; Hobbs, Errett C. ; Olson, L. Karl. / Extracellular acidification parallels insulin secretion in INS-1 and HIT-T15 β-cell lines. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 293, No. 4. pp. 1168-1173.
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