TY - JOUR
T1 - Expression, purification, and properties of the plasma membrane Ca2+ pump and of its N-terminally truncated 105-kDa fragment
AU - Heim, Roger
AU - Iwata, Tomoko
AU - Zvaritch, Elena
AU - Adamo, Hugo P.
AU - Rutishauser, Barbara
AU - Strehler, Emanuel E.
AU - Guerini, Danilo
AU - Carafoli, Ernesto
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1992/12/5
Y1 - 1992/12/5
N2 - Isoform 4b of the human plasma membrane Ca2+ pump was expressed in COS cells and in the baculovirus system (Sf9 cells). A 105-kDa pump fragment lacking the first two transmembrane domains and the socalled transduction domain was also expressed. The expression level was 2-4 times the background in COS cells and at least 7 times in the baculovirus system. Tests on membranes from both systems showed that the expressed pump was active. The expressed pump and the 105-kDa fragment were isolated from Sf9 cell membranes by calmodulin affinity chromatography. The pump had Ca2+-dependent ATPase activity with a calmodulin stimulation factor of 3, formed a La3+-stabilized phosphoenzyme, and had a KM (Ca2+) in the presence of calmodulin of about 1 μM. The 105-kDa fragment, assayed by the phosphoenzyme test on COS or Sf9 cell membranes or by ATPase measurements after isolation from Sf9 cells, proved inactive. Laser confocal microscopy on Sf9 cells showed that both the pump and the 105-kDa fragment were apparently associated with the plasma membrane. The expressed pump in COS and Sf9 cells and the endogenous pump in a number of other cell lines had a slower gel mobility (i.e. a higher apparent molecular mass) than the erythrocyte pump.
AB - Isoform 4b of the human plasma membrane Ca2+ pump was expressed in COS cells and in the baculovirus system (Sf9 cells). A 105-kDa pump fragment lacking the first two transmembrane domains and the socalled transduction domain was also expressed. The expression level was 2-4 times the background in COS cells and at least 7 times in the baculovirus system. Tests on membranes from both systems showed that the expressed pump was active. The expressed pump and the 105-kDa fragment were isolated from Sf9 cell membranes by calmodulin affinity chromatography. The pump had Ca2+-dependent ATPase activity with a calmodulin stimulation factor of 3, formed a La3+-stabilized phosphoenzyme, and had a KM (Ca2+) in the presence of calmodulin of about 1 μM. The 105-kDa fragment, assayed by the phosphoenzyme test on COS or Sf9 cell membranes or by ATPase measurements after isolation from Sf9 cells, proved inactive. Laser confocal microscopy on Sf9 cells showed that both the pump and the 105-kDa fragment were apparently associated with the plasma membrane. The expressed pump in COS and Sf9 cells and the endogenous pump in a number of other cell lines had a slower gel mobility (i.e. a higher apparent molecular mass) than the erythrocyte pump.
UR - http://www.scopus.com/inward/record.url?scp=0026496894&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026496894&partnerID=8YFLogxK
M3 - Article
C2 - 1332959
AN - SCOPUS:0026496894
SN - 0021-9258
VL - 267
SP - 24476
EP - 24484
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 34
ER -