TY - JOUR
T1 - Expression of type VI collagen mRNA during wound healing
AU - Oono, Takashi
AU - Specks, Ulrich
AU - Eckes, Beate
AU - Majewski, Slawomir
AU - Hunzelmann, Nicolas
AU - Timpl, Rupert
AU - Krieg, Thomas
PY - 1993/3
Y1 - 1993/3
N2 - During the highly regulated process of wound healing the expression of the interstitial collagens I and III is increased in a time-dependent fashion. Although ultrastructural and in vitro studies suggest a physiologic role of collagen VI in the organization of extracellular matrix deposition, nothing is known about its role in wound healing. Therefore, we studied collagen VI gene expression during wound healing in humans compared to that of collagens I and III. The presence of specific α1(VI) and α3(VI) mRNA species in scar tissue was demonstrated by Northern blot analysis. Quantification of mRNA expression by dot blot analysis and in situ hybridization indicated that like for the interstitial collagens I and III collagen VI gene expression was increased during wound healing, reaching its maximum 2 weeks after initial insult.In the late phase of wound healing like α1(I) the α1(VI) gene expression was not down regulated significantly. In contrast, a reduction of α3(VI) collagen gene expression was observed as was for the α1(III) collagen gene, indicating a non-coordinate regulation of these chains. Collagen VI gene expression could be localized to fibroblast-like cells and to endothelial cells of newly formed vessels. Collagen VI gene expression was undetectable in smooth muscle cells and myoepithelial cells of eccrine glands. These results indicate that collagen VI gene expression is regulated in a time-dependent fashion and that fibroblasts and endothelial cells appear to play an important role in collagen VI synthesis during wound healing.
AB - During the highly regulated process of wound healing the expression of the interstitial collagens I and III is increased in a time-dependent fashion. Although ultrastructural and in vitro studies suggest a physiologic role of collagen VI in the organization of extracellular matrix deposition, nothing is known about its role in wound healing. Therefore, we studied collagen VI gene expression during wound healing in humans compared to that of collagens I and III. The presence of specific α1(VI) and α3(VI) mRNA species in scar tissue was demonstrated by Northern blot analysis. Quantification of mRNA expression by dot blot analysis and in situ hybridization indicated that like for the interstitial collagens I and III collagen VI gene expression was increased during wound healing, reaching its maximum 2 weeks after initial insult.In the late phase of wound healing like α1(I) the α1(VI) gene expression was not down regulated significantly. In contrast, a reduction of α3(VI) collagen gene expression was observed as was for the α1(III) collagen gene, indicating a non-coordinate regulation of these chains. Collagen VI gene expression could be localized to fibroblast-like cells and to endothelial cells of newly formed vessels. Collagen VI gene expression was undetectable in smooth muscle cells and myoepithelial cells of eccrine glands. These results indicate that collagen VI gene expression is regulated in a time-dependent fashion and that fibroblasts and endothelial cells appear to play an important role in collagen VI synthesis during wound healing.
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U2 - 10.1111/1523-1747.ep12470022
DO - 10.1111/1523-1747.ep12470022
M3 - Article
C2 - 8440917
AN - SCOPUS:0027466950
SN - 0022-202X
VL - 100
SP - 329
EP - 334
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 3
ER -