Expression of the AML-1 oncogene shortens the G1 phase of the cell cycle

David K. Strom, Nip John, Jennifer J Westendorf, Bryan Linggi, Bart Lutterbach, James R. Downing, Noel Lenny, Scott W. Hiebert

Research output: Contribution to journalArticle

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Abstract

The AML-1-encoded transcription factor, AML-1B, regulates numerous hematopoietic-specific genes. Inappropriate expression of AML-1-family proteins is oncogenic in cell culture systems and in mice. To understand the oncogenic functions of AML-1, we established cell lines expressing AML-1B to examine the role of AML-1 in the cell cycle. DNA content analysis and bromodeoxyuridine pulse-chase studies indicated that entry into the S phase of the cell cycle was accelerated by up to 4 h in AML-1B-expressing 32D.3 myeloid progenitor cells as compared with control cells or cells expressing E2F-1. However, AML-1B was not able to induce continued cell cycle progression in the absence of growth factors. The DNA binding and transactivation domains of AML-1B were required for altering the cell cycle. Thus, AML-1B is the first transcription factor that affects the timing of the mammalian cell cycle.

Original languageEnglish (US)
Pages (from-to)3438-3445
Number of pages8
JournalJournal of Biological Chemistry
Volume275
Issue number5
DOIs
StatePublished - Feb 4 2000
Externally publishedYes

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G1 Phase
Oncogenes
Cell Cycle
Cells
Transcription Factors
Myeloid Progenitor Cells
DNA
Bromodeoxyuridine
S Phase
Transcriptional Activation
Intercellular Signaling Peptides and Proteins
Cell Culture Techniques
Cell culture
Cell Line
Genes
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Strom, D. K., John, N., Westendorf, J. J., Linggi, B., Lutterbach, B., Downing, J. R., ... Hiebert, S. W. (2000). Expression of the AML-1 oncogene shortens the G1 phase of the cell cycle. Journal of Biological Chemistry, 275(5), 3438-3445. https://doi.org/10.1074/jbc.275.5.3438

Expression of the AML-1 oncogene shortens the G1 phase of the cell cycle. / Strom, David K.; John, Nip; Westendorf, Jennifer J; Linggi, Bryan; Lutterbach, Bart; Downing, James R.; Lenny, Noel; Hiebert, Scott W.

In: Journal of Biological Chemistry, Vol. 275, No. 5, 04.02.2000, p. 3438-3445.

Research output: Contribution to journalArticle

Strom, DK, John, N, Westendorf, JJ, Linggi, B, Lutterbach, B, Downing, JR, Lenny, N & Hiebert, SW 2000, 'Expression of the AML-1 oncogene shortens the G1 phase of the cell cycle', Journal of Biological Chemistry, vol. 275, no. 5, pp. 3438-3445. https://doi.org/10.1074/jbc.275.5.3438
Strom, David K. ; John, Nip ; Westendorf, Jennifer J ; Linggi, Bryan ; Lutterbach, Bart ; Downing, James R. ; Lenny, Noel ; Hiebert, Scott W. / Expression of the AML-1 oncogene shortens the G1 phase of the cell cycle. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 5. pp. 3438-3445.
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