TY - JOUR
T1 - Expression of the AML-1 oncogene shortens the G1 phase of the cell cycle
AU - Strom, David K.
AU - John, Nip
AU - Westendorf, Jennifer J.
AU - Linggi, Bryan
AU - Lutterbach, Bart
AU - Downing, James R.
AU - Lenny, Noel
AU - Hiebert, Scott W.
PY - 2000/2/4
Y1 - 2000/2/4
N2 - The AML-1-encoded transcription factor, AML-1B, regulates numerous hematopoietic-specific genes. Inappropriate expression of AML-1-family proteins is oncogenic in cell culture systems and in mice. To understand the oncogenic functions of AML-1, we established cell lines expressing AML-1B to examine the role of AML-1 in the cell cycle. DNA content analysis and bromodeoxyuridine pulse-chase studies indicated that entry into the S phase of the cell cycle was accelerated by up to 4 h in AML-1B-expressing 32D.3 myeloid progenitor cells as compared with control cells or cells expressing E2F-1. However, AML-1B was not able to induce continued cell cycle progression in the absence of growth factors. The DNA binding and transactivation domains of AML-1B were required for altering the cell cycle. Thus, AML-1B is the first transcription factor that affects the timing of the mammalian cell cycle.
AB - The AML-1-encoded transcription factor, AML-1B, regulates numerous hematopoietic-specific genes. Inappropriate expression of AML-1-family proteins is oncogenic in cell culture systems and in mice. To understand the oncogenic functions of AML-1, we established cell lines expressing AML-1B to examine the role of AML-1 in the cell cycle. DNA content analysis and bromodeoxyuridine pulse-chase studies indicated that entry into the S phase of the cell cycle was accelerated by up to 4 h in AML-1B-expressing 32D.3 myeloid progenitor cells as compared with control cells or cells expressing E2F-1. However, AML-1B was not able to induce continued cell cycle progression in the absence of growth factors. The DNA binding and transactivation domains of AML-1B were required for altering the cell cycle. Thus, AML-1B is the first transcription factor that affects the timing of the mammalian cell cycle.
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U2 - 10.1074/jbc.275.5.3438
DO - 10.1074/jbc.275.5.3438
M3 - Article
C2 - 10652337
AN - SCOPUS:0034603080
SN - 0021-9258
VL - 275
SP - 3438
EP - 3445
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 5
ER -