Expression of tau exon 8 in different species

Wei Ta Chen, Wan Kyng Liu, Shu Hui Yen

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A synthetic peptide corresponding to a region encoded by tau gene exon 8 was used to raise an antibody. The antibody, E8, was used to probe normal tau from different species and abnormal tau proteins from subjects with Alzheimer's disease. Immunoblotting and enzyme-linked immunosorbent assays demonstrated that only bovine tau reacted with the E8 antibody. The E8 immunoreactive tau isoform was estimated to represent less than 2% of the bovine tau. Our results indicate that bovine tau is unique in containing isoforms positive with E8, and that PHF formation does not require the presence of PHF-tau with exon 8.

Original languageEnglish (US)
Pages (from-to)167-170
Number of pages4
JournalNeuroscience Letters
Volume172
Issue number1-2
DOIs
StatePublished - May 19 1994
Externally publishedYes

Fingerprint

Exons
Antibodies
Protein Isoforms
tau Proteins
Immunoblotting
Alzheimer Disease
Enzyme-Linked Immunosorbent Assay
Peptides
Genes

Keywords

  • Bovine tau
  • Exon 8 expression
  • PHF-tau

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression of tau exon 8 in different species. / Chen, Wei Ta; Liu, Wan Kyng; Yen, Shu Hui.

In: Neuroscience Letters, Vol. 172, No. 1-2, 19.05.1994, p. 167-170.

Research output: Contribution to journalArticle

Chen, Wei Ta ; Liu, Wan Kyng ; Yen, Shu Hui. / Expression of tau exon 8 in different species. In: Neuroscience Letters. 1994 ; Vol. 172, No. 1-2. pp. 167-170.
@article{37b902fca76c405fb95d63aa4330d30b,
title = "Expression of tau exon 8 in different species",
abstract = "A synthetic peptide corresponding to a region encoded by tau gene exon 8 was used to raise an antibody. The antibody, E8, was used to probe normal tau from different species and abnormal tau proteins from subjects with Alzheimer's disease. Immunoblotting and enzyme-linked immunosorbent assays demonstrated that only bovine tau reacted with the E8 antibody. The E8 immunoreactive tau isoform was estimated to represent less than 2{\%} of the bovine tau. Our results indicate that bovine tau is unique in containing isoforms positive with E8, and that PHF formation does not require the presence of PHF-tau with exon 8.",
keywords = "Bovine tau, Exon 8 expression, PHF-tau",
author = "Chen, {Wei Ta} and Liu, {Wan Kyng} and Yen, {Shu Hui}",
year = "1994",
month = "5",
day = "19",
doi = "10.1016/0304-3940(94)90688-2",
language = "English (US)",
volume = "172",
pages = "167--170",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1-2",

}

TY - JOUR

T1 - Expression of tau exon 8 in different species

AU - Chen, Wei Ta

AU - Liu, Wan Kyng

AU - Yen, Shu Hui

PY - 1994/5/19

Y1 - 1994/5/19

N2 - A synthetic peptide corresponding to a region encoded by tau gene exon 8 was used to raise an antibody. The antibody, E8, was used to probe normal tau from different species and abnormal tau proteins from subjects with Alzheimer's disease. Immunoblotting and enzyme-linked immunosorbent assays demonstrated that only bovine tau reacted with the E8 antibody. The E8 immunoreactive tau isoform was estimated to represent less than 2% of the bovine tau. Our results indicate that bovine tau is unique in containing isoforms positive with E8, and that PHF formation does not require the presence of PHF-tau with exon 8.

AB - A synthetic peptide corresponding to a region encoded by tau gene exon 8 was used to raise an antibody. The antibody, E8, was used to probe normal tau from different species and abnormal tau proteins from subjects with Alzheimer's disease. Immunoblotting and enzyme-linked immunosorbent assays demonstrated that only bovine tau reacted with the E8 antibody. The E8 immunoreactive tau isoform was estimated to represent less than 2% of the bovine tau. Our results indicate that bovine tau is unique in containing isoforms positive with E8, and that PHF formation does not require the presence of PHF-tau with exon 8.

KW - Bovine tau

KW - Exon 8 expression

KW - PHF-tau

UR - http://www.scopus.com/inward/record.url?scp=0028343029&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028343029&partnerID=8YFLogxK

U2 - 10.1016/0304-3940(94)90688-2

DO - 10.1016/0304-3940(94)90688-2

M3 - Article

C2 - 8084527

AN - SCOPUS:0028343029

VL - 172

SP - 167

EP - 170

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 1-2

ER -