Expression of tau exon 8 in different species

Wei Ta Chen, Wan Kyng Liu, Shu Hui Yen

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

A synthetic peptide corresponding to a region encoded by tau gene exon 8 was used to raise an antibody. The antibody, E8, was used to probe normal tau from different species and abnormal tau proteins from subjects with Alzheimer's disease. Immunoblotting and enzyme-linked immunosorbent assays demonstrated that only bovine tau reacted with the E8 antibody. The E8 immunoreactive tau isoform was estimated to represent less than 2% of the bovine tau. Our results indicate that bovine tau is unique in containing isoforms positive with E8, and that PHF formation does not require the presence of PHF-tau with exon 8.

Original languageEnglish (US)
Pages (from-to)167-170
Number of pages4
JournalNeuroscience Letters
Volume172
Issue number1-2
DOIs
StatePublished - May 19 1994
Externally publishedYes

    Fingerprint

Keywords

  • Bovine tau
  • Exon 8 expression
  • PHF-tau

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this