Expression of natural killer receptors in T- and NK-cells

comparison of healthy individuals, patients with prior stem cell transplant, and patients undergoing chemotherapy.

Horatiu Olteanu, Bernard C. Schur, Christopher Bredeson, Ehab Atallah, Steven H. Kroft

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We studied the expression of natural killer receptors (NKRs) on peripheral blood cytotoxic T lymphocytes and NK cells in patients who underwent an allogeneic stem cell transplant (SCT), and compared these findings with results from healthy individuals (CTRL) and patients undergoing chemotherapy (CHEMO), respectively. Peripheral blood mononuclear cells were analyzed by flow cytometry with antibodies against the NKRs CD158a, CD158b, CD158e (known as killer immunoglobulin-like receptors, KIRs), and CD94. Expression of NKRs was evaluated separately in CD56+, CD57+, and CD56/CD57 (double+) subsets of T and NK cells. We found mainly differences in CD158a and CD94 expression between the three cohorts, with the SCT and CHEMO groups usually showing similar changes, when compared to the CTRL population. None of the patients with SCT or CHEMO demonstrated patterns of restricted NKR expression. Our results provide a comprehensive overview of KIR and CD94 expression in T and NK cells following SCT and chemotherapy.

Original languageEnglish (US)
Pages (from-to)481-487
Number of pages7
JournalLeukemia & lymphoma
Volume51
Issue number3
DOIs
StatePublished - Mar 1 2010
Externally publishedYes

Fingerprint

Natural Killer Cells
Stem Cells
KIR Receptors
Transplants
Drug Therapy
T-Lymphocytes
Cytotoxic T-Lymphocytes
Blood Cells
Flow Cytometry
Antibodies
Population

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Expression of natural killer receptors in T- and NK-cells : comparison of healthy individuals, patients with prior stem cell transplant, and patients undergoing chemotherapy. / Olteanu, Horatiu; Schur, Bernard C.; Bredeson, Christopher; Atallah, Ehab; Kroft, Steven H.

In: Leukemia & lymphoma, Vol. 51, No. 3, 01.03.2010, p. 481-487.

Research output: Contribution to journalArticle

@article{656ae750f558434b8a4f435137be05e7,
title = "Expression of natural killer receptors in T- and NK-cells: comparison of healthy individuals, patients with prior stem cell transplant, and patients undergoing chemotherapy.",
abstract = "We studied the expression of natural killer receptors (NKRs) on peripheral blood cytotoxic T lymphocytes and NK cells in patients who underwent an allogeneic stem cell transplant (SCT), and compared these findings with results from healthy individuals (CTRL) and patients undergoing chemotherapy (CHEMO), respectively. Peripheral blood mononuclear cells were analyzed by flow cytometry with antibodies against the NKRs CD158a, CD158b, CD158e (known as killer immunoglobulin-like receptors, KIRs), and CD94. Expression of NKRs was evaluated separately in CD56+, CD57+, and CD56/CD57 (double+) subsets of T and NK cells. We found mainly differences in CD158a and CD94 expression between the three cohorts, with the SCT and CHEMO groups usually showing similar changes, when compared to the CTRL population. None of the patients with SCT or CHEMO demonstrated patterns of restricted NKR expression. Our results provide a comprehensive overview of KIR and CD94 expression in T and NK cells following SCT and chemotherapy.",
author = "Horatiu Olteanu and Schur, {Bernard C.} and Christopher Bredeson and Ehab Atallah and Kroft, {Steven H.}",
year = "2010",
month = "3",
day = "1",
doi = "10.3109/10428190903552120",
language = "English (US)",
volume = "51",
pages = "481--487",
journal = "Leukemia and Lymphoma",
issn = "1042-8194",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - Expression of natural killer receptors in T- and NK-cells

T2 - comparison of healthy individuals, patients with prior stem cell transplant, and patients undergoing chemotherapy.

AU - Olteanu, Horatiu

AU - Schur, Bernard C.

AU - Bredeson, Christopher

AU - Atallah, Ehab

AU - Kroft, Steven H.

PY - 2010/3/1

Y1 - 2010/3/1

N2 - We studied the expression of natural killer receptors (NKRs) on peripheral blood cytotoxic T lymphocytes and NK cells in patients who underwent an allogeneic stem cell transplant (SCT), and compared these findings with results from healthy individuals (CTRL) and patients undergoing chemotherapy (CHEMO), respectively. Peripheral blood mononuclear cells were analyzed by flow cytometry with antibodies against the NKRs CD158a, CD158b, CD158e (known as killer immunoglobulin-like receptors, KIRs), and CD94. Expression of NKRs was evaluated separately in CD56+, CD57+, and CD56/CD57 (double+) subsets of T and NK cells. We found mainly differences in CD158a and CD94 expression between the three cohorts, with the SCT and CHEMO groups usually showing similar changes, when compared to the CTRL population. None of the patients with SCT or CHEMO demonstrated patterns of restricted NKR expression. Our results provide a comprehensive overview of KIR and CD94 expression in T and NK cells following SCT and chemotherapy.

AB - We studied the expression of natural killer receptors (NKRs) on peripheral blood cytotoxic T lymphocytes and NK cells in patients who underwent an allogeneic stem cell transplant (SCT), and compared these findings with results from healthy individuals (CTRL) and patients undergoing chemotherapy (CHEMO), respectively. Peripheral blood mononuclear cells were analyzed by flow cytometry with antibodies against the NKRs CD158a, CD158b, CD158e (known as killer immunoglobulin-like receptors, KIRs), and CD94. Expression of NKRs was evaluated separately in CD56+, CD57+, and CD56/CD57 (double+) subsets of T and NK cells. We found mainly differences in CD158a and CD94 expression between the three cohorts, with the SCT and CHEMO groups usually showing similar changes, when compared to the CTRL population. None of the patients with SCT or CHEMO demonstrated patterns of restricted NKR expression. Our results provide a comprehensive overview of KIR and CD94 expression in T and NK cells following SCT and chemotherapy.

UR - http://www.scopus.com/inward/record.url?scp=79954619318&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79954619318&partnerID=8YFLogxK

U2 - 10.3109/10428190903552120

DO - 10.3109/10428190903552120

M3 - Article

VL - 51

SP - 481

EP - 487

JO - Leukemia and Lymphoma

JF - Leukemia and Lymphoma

SN - 1042-8194

IS - 3

ER -