Abstract
Latent transforming growth factor-β binding protein-1 (LTBP1) has been implicated in the control of secretion, localization, and activation of TGFβ (transforming growth factor-β). We developed a quantitative reverse-transcriptase polymerase chain reaction (Q-RT-PCR) assay using an RNA internal standard to examine the expression of three alternatively spliced isoforms of LTBP1 (LTBP1Δ41, LTBP1Δ53, and LTBP1Δ55) in a variety of human tissues. The assays were also used to determine the expression of LTBP1L and LTBP1S isoforms and total LTBP1. The Q-RT-PCR assays were highly reproducible and showed that in most tissues LTBP1Δ55 and LTBP1L were minor components of LTBP1. The proportion of LTBP1Δ41 ranged from 2% of total LTBP1 mRNA in early coronary atherosclerotic lesions to 54% in advanced lesions.
Original language | English (US) |
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Pages (from-to) | 213-225 |
Number of pages | 13 |
Journal | Biochemical Genetics |
Volume | 49 |
Issue number | 3-4 |
DOIs | |
State | Published - Apr 2011 |
Keywords
- Alternative splicing
- Genetic variation
- Latent transforming growth factor-β binding protein
- Q-RT-PCR
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Biochemistry
- Molecular Biology
- Genetics