Expression of Mas-related gene X2 on mast cells is upregulated in the skin of patients with severe chronic urticaria

Daisuke Fujisawa, Jun Ichi Kashiwakura, Hirohito Kita, Yusuke Kikukawa, Yasushi Fujitani, Tomomi Sasaki-Sakamoto, Kazumichi Kuroda, Satoshi Nunomura, Koremasa Hayama, Tadashi Terui, Chisei Ra, Yoshimichi Okayama

Research output: Contribution to journalArticle

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Abstract

RESULTS: The number of MrgX2(+) skin MCs and the percentage of MrgX2(+) MCs in all MCs in patients with CU were significantly greater than those in NC subjects. Eosinophil infiltration in urticarial lesions was observed in 7 of 9 patients with CU. SP, major basic protein, and eosinophil peroxidase, but not eosinophil-derived neurotoxin, induced histamine release from human skin MCs through MrgX2.

CONCLUSION: MrgX2 might be a new target molecule for the treatment of wheal reactions in patients with severe CU.

BACKGROUND: Wheal reactions to intradermally injected neuropeptides, such as substance P (SP) and vasoactive intestinal peptide, are significantly larger and longer lasting in patients with chronic urticaria (CU) than in nonatopic control (NC) subjects. Mas-related gene X2 (MrgX2) has been identified as a receptor for basic neuropeptides, such as SP and vasoactive intestinal peptide. Mast cell (MC) responsiveness to eosinophil mediators contributes to the late-phase reaction of allergy.

OBJECTIVE: We sought to compare the frequency of MrgX2 expression in skin MCs from patients with CU and NC subjects and to identify the receptor for basic eosinophil granule proteins on human skin MCs.

METHODS: MrgX2 expression was investigated by using immunofluorescence in skin tissues from NC subjects and patients with severe CU and on skin-derived cultured MCs. MrgX2 expression in human MCs was reduced by using a lentiviral small hairpin RNA silencing technique. Ca(2+) influx was measured in CHO cells transfected with MrgX2 in response to eosinophil granule proteins. Histamine and prostaglandin D2 levels were measured by using enzyme immunoassays.

Original languageEnglish (US)
Pages (from-to)622-633
Number of pages12
JournalThe Journal of allergy and clinical immunology
Volume134
Issue number3
DOIs
StatePublished - Sep 1 2014

Fingerprint

Urticaria
Mast Cells
Skin
Eosinophil Granule Proteins
Substance P
Genes
Vasoactive Intestinal Peptide
Gene Expression
Eosinophils
Eosinophil-Derived Neurotoxin
Eosinophil Peroxidase
Neuropeptide Receptors
Prostaglandin D2
CHO Cells
Histamine Release
RNA Interference
Neuropeptides
Immunoenzyme Techniques
Histamine
Small Interfering RNA

Keywords

  • Chronic urticaria
  • eosinophil granule proteins
  • eosinophil peroxidase
  • eosinophils
  • histamine
  • human skin mast cells
  • major basic protein
  • Mas-related gene X2
  • substance P

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Expression of Mas-related gene X2 on mast cells is upregulated in the skin of patients with severe chronic urticaria. / Fujisawa, Daisuke; Kashiwakura, Jun Ichi; Kita, Hirohito; Kikukawa, Yusuke; Fujitani, Yasushi; Sasaki-Sakamoto, Tomomi; Kuroda, Kazumichi; Nunomura, Satoshi; Hayama, Koremasa; Terui, Tadashi; Ra, Chisei; Okayama, Yoshimichi.

In: The Journal of allergy and clinical immunology, Vol. 134, No. 3, 01.09.2014, p. 622-633.

Research output: Contribution to journalArticle

Fujisawa, D, Kashiwakura, JI, Kita, H, Kikukawa, Y, Fujitani, Y, Sasaki-Sakamoto, T, Kuroda, K, Nunomura, S, Hayama, K, Terui, T, Ra, C & Okayama, Y 2014, 'Expression of Mas-related gene X2 on mast cells is upregulated in the skin of patients with severe chronic urticaria', The Journal of allergy and clinical immunology, vol. 134, no. 3, pp. 622-633. https://doi.org/10.1016/j.jaci.2014.05.004
Fujisawa, Daisuke ; Kashiwakura, Jun Ichi ; Kita, Hirohito ; Kikukawa, Yusuke ; Fujitani, Yasushi ; Sasaki-Sakamoto, Tomomi ; Kuroda, Kazumichi ; Nunomura, Satoshi ; Hayama, Koremasa ; Terui, Tadashi ; Ra, Chisei ; Okayama, Yoshimichi. / Expression of Mas-related gene X2 on mast cells is upregulated in the skin of patients with severe chronic urticaria. In: The Journal of allergy and clinical immunology. 2014 ; Vol. 134, No. 3. pp. 622-633.
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abstract = "RESULTS: The number of MrgX2(+) skin MCs and the percentage of MrgX2(+) MCs in all MCs in patients with CU were significantly greater than those in NC subjects. Eosinophil infiltration in urticarial lesions was observed in 7 of 9 patients with CU. SP, major basic protein, and eosinophil peroxidase, but not eosinophil-derived neurotoxin, induced histamine release from human skin MCs through MrgX2.CONCLUSION: MrgX2 might be a new target molecule for the treatment of wheal reactions in patients with severe CU.BACKGROUND: Wheal reactions to intradermally injected neuropeptides, such as substance P (SP) and vasoactive intestinal peptide, are significantly larger and longer lasting in patients with chronic urticaria (CU) than in nonatopic control (NC) subjects. Mas-related gene X2 (MrgX2) has been identified as a receptor for basic neuropeptides, such as SP and vasoactive intestinal peptide. Mast cell (MC) responsiveness to eosinophil mediators contributes to the late-phase reaction of allergy.OBJECTIVE: We sought to compare the frequency of MrgX2 expression in skin MCs from patients with CU and NC subjects and to identify the receptor for basic eosinophil granule proteins on human skin MCs.METHODS: MrgX2 expression was investigated by using immunofluorescence in skin tissues from NC subjects and patients with severe CU and on skin-derived cultured MCs. MrgX2 expression in human MCs was reduced by using a lentiviral small hairpin RNA silencing technique. Ca(2+) influx was measured in CHO cells transfected with MrgX2 in response to eosinophil granule proteins. Histamine and prostaglandin D2 levels were measured by using enzyme immunoassays.",
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T1 - Expression of Mas-related gene X2 on mast cells is upregulated in the skin of patients with severe chronic urticaria

AU - Fujisawa, Daisuke

AU - Kashiwakura, Jun Ichi

AU - Kita, Hirohito

AU - Kikukawa, Yusuke

AU - Fujitani, Yasushi

AU - Sasaki-Sakamoto, Tomomi

AU - Kuroda, Kazumichi

AU - Nunomura, Satoshi

AU - Hayama, Koremasa

AU - Terui, Tadashi

AU - Ra, Chisei

AU - Okayama, Yoshimichi

PY - 2014/9/1

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N2 - RESULTS: The number of MrgX2(+) skin MCs and the percentage of MrgX2(+) MCs in all MCs in patients with CU were significantly greater than those in NC subjects. Eosinophil infiltration in urticarial lesions was observed in 7 of 9 patients with CU. SP, major basic protein, and eosinophil peroxidase, but not eosinophil-derived neurotoxin, induced histamine release from human skin MCs through MrgX2.CONCLUSION: MrgX2 might be a new target molecule for the treatment of wheal reactions in patients with severe CU.BACKGROUND: Wheal reactions to intradermally injected neuropeptides, such as substance P (SP) and vasoactive intestinal peptide, are significantly larger and longer lasting in patients with chronic urticaria (CU) than in nonatopic control (NC) subjects. Mas-related gene X2 (MrgX2) has been identified as a receptor for basic neuropeptides, such as SP and vasoactive intestinal peptide. Mast cell (MC) responsiveness to eosinophil mediators contributes to the late-phase reaction of allergy.OBJECTIVE: We sought to compare the frequency of MrgX2 expression in skin MCs from patients with CU and NC subjects and to identify the receptor for basic eosinophil granule proteins on human skin MCs.METHODS: MrgX2 expression was investigated by using immunofluorescence in skin tissues from NC subjects and patients with severe CU and on skin-derived cultured MCs. MrgX2 expression in human MCs was reduced by using a lentiviral small hairpin RNA silencing technique. Ca(2+) influx was measured in CHO cells transfected with MrgX2 in response to eosinophil granule proteins. Histamine and prostaglandin D2 levels were measured by using enzyme immunoassays.

AB - RESULTS: The number of MrgX2(+) skin MCs and the percentage of MrgX2(+) MCs in all MCs in patients with CU were significantly greater than those in NC subjects. Eosinophil infiltration in urticarial lesions was observed in 7 of 9 patients with CU. SP, major basic protein, and eosinophil peroxidase, but not eosinophil-derived neurotoxin, induced histamine release from human skin MCs through MrgX2.CONCLUSION: MrgX2 might be a new target molecule for the treatment of wheal reactions in patients with severe CU.BACKGROUND: Wheal reactions to intradermally injected neuropeptides, such as substance P (SP) and vasoactive intestinal peptide, are significantly larger and longer lasting in patients with chronic urticaria (CU) than in nonatopic control (NC) subjects. Mas-related gene X2 (MrgX2) has been identified as a receptor for basic neuropeptides, such as SP and vasoactive intestinal peptide. Mast cell (MC) responsiveness to eosinophil mediators contributes to the late-phase reaction of allergy.OBJECTIVE: We sought to compare the frequency of MrgX2 expression in skin MCs from patients with CU and NC subjects and to identify the receptor for basic eosinophil granule proteins on human skin MCs.METHODS: MrgX2 expression was investigated by using immunofluorescence in skin tissues from NC subjects and patients with severe CU and on skin-derived cultured MCs. MrgX2 expression in human MCs was reduced by using a lentiviral small hairpin RNA silencing technique. Ca(2+) influx was measured in CHO cells transfected with MrgX2 in response to eosinophil granule proteins. Histamine and prostaglandin D2 levels were measured by using enzyme immunoassays.

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KW - eosinophil granule proteins

KW - eosinophil peroxidase

KW - eosinophils

KW - histamine

KW - human skin mast cells

KW - major basic protein

KW - Mas-related gene X2

KW - substance P

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