EXPRESSION OF I‐Aαk AND ALLELIC RESTRICTION ON Aα/Aα PAIRING IN TRANSGENIC MICE

J. Martin, B. ‐Y Wei, S. Savarirayan, C. S. David

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Cell surface expression of class II major histocompatibility complex encoded (la) molecules depends on association of the component α and β chain into a stable heterodimer. Studies on cell lines transfected with MHC class II genes have revealed important limitations on the assembly of haplotype‐mismatched Aα:Aβ complex. In order to study α: β chain pairing restrictions in vivo a number of lines of transgenic mice carrying the Aαk gene were generated. The transgene was studied in the context of H‐2b, H‐2s, H‐24, H‐2u, H‐2f and H‐2v haplotypes. Initial FACS analysis of spleen and peripheral blood cells showed no Aαk expression. Spleen cells stimulated with LPS and analysed by FACS showed Aαk expression in three different H‐2b strains as well as H‐2u, but not in others. These data indicate that Aαk can associate with Aβb and Aαk, but not with Aβs,Aβq, Aβf, Aβv.

Original languageEnglish (US)
Pages (from-to)151-159
Number of pages9
JournalInternational Journal of Immunogenetics
Volume17
Issue number1-2
DOIs
StatePublished - Feb 1990

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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