Cell surface expression of class II major histocompatibility complex encoded (la) molecules depends on association of the component α and β chain into a stable heterodimer. Studies on cell lines transfected with MHC class II genes have revealed important limitations on the assembly of haplotype‐mismatched Aα:Aβ complex. In order to study α: β chain pairing restrictions in vivo a number of lines of transgenic mice carrying the Aαk gene were generated. The transgene was studied in the context of H‐2b, H‐2s, H‐24, H‐2u, H‐2f and H‐2v haplotypes. Initial FACS analysis of spleen and peripheral blood cells showed no Aαk expression. Spleen cells stimulated with LPS and analysed by FACS showed Aαk expression in three different H‐2b strains as well as H‐2u, but not in others. These data indicate that Aαk can associate with Aβb and Aαk, but not with Aβs,Aβq, Aβf, Aβv.
|Original language||English (US)|
|Number of pages||9|
|Journal||International Journal of Immunogenetics|
|State||Published - Feb 1990|
ASJC Scopus subject areas
- Molecular Biology